6UPO

Crystal structure of H334A variant of cytosolic fumarate hydratase from Leishmania major in a complex with S-malate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Fumarate hydratase 2	polymer	604	66446.4	2	UniProt (E9AE57) Pfam (PF05681) Pfam (PF05683) In PDB	Leishmania major strain Friedlin	LmFH-2
2	A, B	IRON/SULFUR CLUSTER	non-polymer		351.6	2	Chemie (SF4)
3	A, B	(2S)-2-hydroxybutanedioic acid	non-polymer		134.1	2	Chemie (LMR)
4		water	water		18.0	12	Chemie (HOH)

Sequence modifications

A, B: 1 - 568 (UniProt: E9AE57)

PDB	External Database	Details
Met -35	-	expression tag
Gly -34	-	expression tag
Ser -33	-	expression tag
Ser -32	-	expression tag
His -31	-	expression tag
His -30	-	expression tag
His -29	-	expression tag
His -28	-	expression tag
His -27	-	expression tag
His -26	-	expression tag
Ser -25	-	expression tag
Ser -24	-	expression tag
Gly -23	-	expression tag
Leu -22	-	expression tag
Val -21	-	expression tag
Pro -20	-	expression tag
Arg -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
Met -15	-	expression tag
Ala -14	-	expression tag
Ser -13	-	expression tag
Met -12	-	expression tag
Thr -11	-	expression tag
Gly -10	-	expression tag
Gly -9	-	expression tag
Gln -8	-	expression tag
Gln -7	-	expression tag
Met -6	-	expression tag
Gly -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Glu -1	-	expression tag
Phe 0	-	expression tag
Ala 334	His 334	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	132892.8
Non-Polymers*	Number of molecules	4
	Total formula weight	971.5
All*	Total formula weight	133864.3

*Water molecules are not included.