6UM7
Cryo-EM structure of vaccine-elicited HIV-1 neutralizing antibody DH270.mu1 in complex with CH848 10.17DT Env
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, F, L (A, E, I) | Envelope glycoprotein gp120 | polymer | 463 | 51736.5 | 3 | UniProt (A0A1W6IPB2) Pfam (PF00516) | Human immunodeficiency virus 1 | Env polyprotein |
| 2 | B, E, I (H, D, 1) | Envelope glycoprotein gp41 | polymer | 161 | 18245.8 | 3 | UniProt (Q2N0S7) Pfam (PF00517) | Human immunodeficiency virus 1 | Env polyprotein |
| 3 | C, G, J (J, F, B) | DH270.mu1 Fab Heavy Chain | polymer | 251 | 26975.3 | 3 | Homo sapiens (Human) | ||
| 4 | D, H, K (K, G, C) | DH270.mu1 Fab Light chain | polymer | 235 | 24781.7 | 3 | Homo sapiens (Human) | ||
| 5 | AA, M, N, P, Q... (Z, L, M, O, P...) | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 12 | In PDB GlyTouCan (G15407YE) | |||
| 6 | O, T, Y (N, S, X) | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1073.0 | 3 | In PDB GlyTouCan (G56014GC) | |||
| 7 | BA, CA, DA, EA, FA... (A, E, I) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 24 | Chemie (NAG) |
Sequence modifications
A, E, I: 34 - 505 (UniProt: A0A1W6IPB2)
H, D, 1: 511 - 664 (UniProt: Q2N0S7)
| PDB | External Database | Details |
|---|---|---|
| Ala 31 | - | expression tag |
| Glu 32 | - | expression tag |
| Asn 33 | - | expression tag |
| Asp 133 | Asn 129 | conflict |
| Thr 138 | Asn 134 | conflict |
| Cys 201 | Val 189 | conflict |
| Cys 433 | Ala 417 | conflict |
| Lys 490 | Glu 474 | conflict |
| Glu 492 | Gln 476 | conflict |
| Val 496 | Ile 480 | conflict |
| Arg 500 | Gly 484 | conflict |
| Cys 501 | Ala 485 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Val 504 | - | expression tag |
| Gly 505 | - | expression tag |
| Arg 506 | - | expression tag |
| Arg 507 | - | expression tag |
| Arg 508 | - | expression tag |
| Arg 509 | - | expression tag |
| Arg 510 | - | expression tag |
| Pro 559 | Ile 556 | conflict |
| Cys 605 | Thr 602 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 365217.9 | |
| Branched | Number of molecules | 15 |
| Total formula weight | 10257.4 | |
| Non-Polymers* | Number of molecules | 24 |
| Total formula weight | 5309.0 | |
| All* | Total formula weight | 380784.3 |
