6UM5
Cryo-EM structure of HIV-1 neutralizing antibody DH270 UCA3 in complex with CH848 10.17DT Env
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, E, I | CH848 10.17 DT gp120 | polymer | 462 | 51693.5 | 3 | UniProt (A0A1W6IPB2) Pfam (PF00516) In PDB | Human immunodeficiency virus 1 | Env polyprotein |
| 2 | B, F, J | Envelope glycoprotein gp160 | polymer | 153 | 17162.5 | 3 | UniProt (Q2N0S7) Pfam (PF00517) In PDB | Human immunodeficiency virus 1 | Env polyprotein |
| 3 | C, G, K | DH270 UCA3 Fab Heavy Chain | polymer | 238 | 25779.8 | 3 | Homo sapiens (Human) | ||
| 4 | D, H, L | DH270 UCA3 Fab Light Chain | polymer | 216 | 22648.0 | 3 | Homo sapiens (Human) | ||
| 5 | a, e, g, M, Q... | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 9 | In PDB GlyTouCan (G42666HT) | |||
| 6 | b, N, U | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1235.1 | 3 | In PDB GlyTouCan (G27327VV) | |||
| 7 | c, O, V | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 789.7 | 3 | In PDB GlyTouCan (G95663PY) | |||
| 8 | d, f, P, R, W... | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 6 | In PDB GlyTouCan (G15407YE) | |||
| 9 | I, A, E | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 27 | Chemie (NAG) |
Sequence modifications
A, E, I: 34 - 505 (UniProt: A0A1W6IPB2)
B, F, J: 512 - 664 (UniProt: Q2N0S7)
| PDB | External Database | Details |
|---|---|---|
| Glu 32 | - | expression tag |
| Asn 33 | - | expression tag |
| Asp 133 | Asn 129 | conflict |
| Thr 138 | Asn 134 | conflict |
| Cys 200 | Ala 188 | conflict |
| Cys 433 | Ala 417 | conflict |
| Lys 490 | Glu 474 | conflict |
| Glu 492 | Gln 476 | conflict |
| Val 496 | Ile 480 | conflict |
| Arg 500 | Gly 484 | conflict |
| Cys 501 | Ala 485 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Cys 605 | Thr 602 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 351851.6 | |
| Branched | Number of molecules | 21 |
| Total formula weight | 13413.4 | |
| Non-Polymers* | Number of molecules | 27 |
| Total formula weight | 5972.6 | |
| All* | Total formula weight | 371237.6 |
