6UK5

Structure of SAM bound CalS10, an amino pentose methyltransferase from Micromonospora echinaspora involved in calicheamicin biosynthesis

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	CalS10	polymer	261	28631.3	2	UniProt (Q8KNF4)	Micromonospora echinospora
2	C, G (A, B)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG) PubChem (8117)
3	D, H (A, B)	S-ADENOSYLMETHIONINE	non-polymer		398.4	2	Chemie (SAM) PubChem (5136) PubChem (34755) PubChem (58633578) PubChem (60038769) PubChem (73897310) PubChem (58766358) PubChem (87224684) PubChem (131697735) PubChem (9865603) PubChem (145712080) PubChem (12312798) PubChem (132988922) PubChem (134692588) PubChem (135030730) PubChem (140459785) PubChem (163046094)
4	E, I (A, B)	ACETATE ION	non-polymer		59.0	2	Chemie (ACT) PubChem (175)
5	F, J (A, B)	PENTAETHYLENE GLYCOL	non-polymer		238.3	2	Chemie (1PE) PubChem (62551)
6	K, L (A, B)	water	water		18.0	26	Chemie (HOH)

Sequence modifications

A, B: 1 - 245 (UniProt: Q8KNF4)

PDB	External Database	Details
Gly -15	-	expression tag
Ser -14	-	expression tag
Ser -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	57262.6
Non-Polymers*	Number of molecules	8
	Total formula weight	1603.8
All*	Total formula weight	58866.4

*Water molecules are not included.