6UFZ

Crystal structure of a GH128 (subgroup I) endo-beta-1,3-glucanase (E199Q mutant) from Amycolatopsis mediterranei (AmGH128_I)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Glyco_hydro_cc domain-containing protein	polymer	263	27916.9	1	UniProt (G0FQ07) In PDB	Amycolatopsis mediterranei
2		water	water		18.0	113	Chemie (HOH)

Sequence modifications

A: 28 - 269 (UniProt: G0FQ07)

PDB	External Database	Details
Met 7	-	initiating methionine
Gly 8	-	expression tag
Ser 9	-	expression tag
Ser 10	-	expression tag
His 11	-	expression tag
His 12	-	expression tag
His 13	-	expression tag
His 14	-	expression tag
His 15	-	expression tag
His 16	-	expression tag
Ser 17	-	expression tag
Ser 18	-	expression tag
Gly 19	-	expression tag
Leu 20	-	expression tag
Val 21	-	expression tag
Pro 22	-	expression tag
Ala 23	-	expression tag
Gly 24	-	expression tag
Ser 25	-	expression tag
His 26	-	expression tag
Met 27	-	expression tag
Gln 199	Glu 199	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	27916.9
All*	Total formula weight	27916.9

*Water molecules are not included.