6UEU
Crystal structure of BF DNA polymerase F710Y mutant bound to tetrahydrofuran and dATP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, D) | DNA polymerase I | polymer | 580 | 66144.8 | 2 | UniProt (D9N168) Pfam (PF22619) Pfam (PF00476) | Geobacillus stearothermophilus | |
| 2 | C, E (B, E) | DNA (5'-D(*CP*GP*AP*TP*CP*AP*CP*GP*(2DT))-3') | polymer | 9 | 2699.8 | 2 | synthetic construct | ||
| 3 | D, F (C, F) | DNA (5'-D(*CP*GP*(3DR)P*AP*CP*GP*TP*GP*AP*TP*CP*G)-3') | polymer | 12 | 3554.3 | 2 | synthetic construct | ||
| 4 | G, H (G, H) | beta-D-fructofuranose-(2-1)-alpha-D-glucopyranose | branched | 342.3 | 2 | In PDB BIRD (PRD_900003) | sucrose | ||
| 5 | I, J (A, D) | 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE | non-polymer | 491.2 | 2 | Chemie (DTP) | |||
| 6 | K (D) | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) | |||
| 7 | L (D) | (4S)-2-METHYL-2,4-PENTANEDIOL | non-polymer | 118.2 | 1 | Chemie (MPD) | |||
| 8 | M, N, O, P, Q... (A, D, B, C, E...) | water | water | 18.0 | 865 | Chemie (HOH) |
Sequence modifications
A, D: 298 - 876 (UniProt: D9N168)
| PDB | External Database | Details |
|---|---|---|
| Ala 297 | - | expression tag |
| Tyr 710 | Phe 413 | engineered mutation |
| Val 713 | Pro 416 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 144797.9 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 684.6 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 1196.6 | |
| All* | Total formula weight | 146679.1 |
