6UER

Crystal form 2: Structure of TBP bound to C-C mismatch at pH 7

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, B)	TATA-box-binding protein 1	polymer	219	24514.7	2	UniProt (P28147) Pfam (PF00352)	Arabidopsis thaliana (Mouse-ear cress)	AtTBP1,TATA sequence-binding protein 1,TBP-1,TATA-binding factor 1,TATA-box factor 1,Transcription initiation factor TFIID TBP-1 subunit
2	B, E (C, E)	DNA (5'-D(*GP*CP*TP*AP*TP*AP*AP*AP*CP*GP*GP*GP*CP*A)-3')	polymer	14	4313.8	2		synthetic construct
3	C, F (D, F)	DNA (5'-D(*TP*GP*CP*CP*CP*CP*TP*TP*TP*AP*TP*AP*GP*C)-3')	polymer	14	4206.7	2		synthetic construct
4	G, H, I, J (A, C, D, F)	water	water		18.0	9	Chemie (HOH)

Sequence modifications

A, B: 1 - 200 (UniProt: P28147)

PDB	External Database	Details
Met -18	-	initiating methionine
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	66070.5
All*	Total formula weight	66070.5

*Water molecules are not included.