6UB6

Crystal structure of a GH128 (subgroup IV) endo-beta-1,3-glucanase from Lentinula edodes (LeGH128_IV) in complex with laminaritetraose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Endo-beta-1,3-glucanase	polymer	270	28780.8	1	UniProt (G9M5R4) Pfam (PF11790) In PDB	Lentinula edodes (Shiitake mushroom)
2	B	beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose	branched		666.6	1	In PDB GlyTouCan (G87841AX)
3	C	beta-D-glucopyranose-(1-3)-beta-D-glucopyranose	branched		342.3	1	In PDB BIRD (PRD_900024) GlyTouCan (G36535HU)		beta-laminaribiose
4	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
5		water	water		18.0	383	Chemie (HOH)

Sequence modifications

A: 21 - 267 (UniProt: G9M5R4)

PDB	External Database	Details
Met -2	-	initiating methionine
Gly -1	-	expression tag
Ser 0	-	expression tag
Ser 1	-	expression tag
His 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
Ser 8	-	expression tag
Ser 9	-	expression tag
Gly 10	-	expression tag
Leu 11	-	expression tag
Val 12	-	expression tag
Pro 13	-	expression tag
Arg 14	-	expression tag
Gly 15	-	expression tag
Ser 16	-	expression tag
His 17	-	expression tag
Met 18	-	expression tag
Ala 19	-	expression tag
Ser 20	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	28780.8
Branched	Number of molecules	2
	Total formula weight	1008.9
Non-Polymers*	Number of molecules	1
	Total formula weight	35.5
All*	Total formula weight	29825.2

*Water molecules are not included.