6UB4

Crystal structure (C2 form) of a GH128 (subgroup IV) endo-beta-1,3-glucanase from Lentinula edodes (LeGH128_IV) in complex with laminaritriose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	GLYCOSIDE HYDROLASE	polymer	270	28780.8	2	UniProt (G9M5R4) Pfam (PF11790)	Lentinula edodes (Shiitake mushroom)
2	C (C)	beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose	branched		504.4	1	In PDB GlyTouCan (G00024MO)
3	D, E (D, E)	beta-D-glucopyranose-(1-3)-beta-D-glucopyranose	branched		342.3	2	In PDB BIRD (PRD_900024) GlyTouCan (G36535HU)		beta-laminaribiose
4	F, G (A, B)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
5	H (B)	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	1	Chemie (MES)
6	I, J (A, B)	water	water		18.0	640	Chemie (HOH)

Sequence modifications

A, B: 21 - 267 (UniProt: G9M5R4)

PDB	External Database	Details
Met -2	-	initiating methionine
Gly -1	-	expression tag
Ser 0	-	expression tag
Ser 1	-	expression tag
His 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
Ser 8	-	expression tag
Ser 9	-	expression tag
Gly 10	-	expression tag
Leu 11	-	expression tag
Val 12	-	expression tag
Pro 13	-	expression tag
Arg 14	-	expression tag
Gly 15	-	expression tag
Ser 16	-	expression tag
His 17	-	expression tag
Met 18	-	expression tag
Ala 19	-	expression tag
Ser 20	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	57561.7
Branched	Number of molecules	3
	Total formula weight	1189.0
Non-Polymers*	Number of molecules	3
	Total formula weight	266.1
All*	Total formula weight	59016.8

*Water molecules are not included.