6UAU

Crystal structure of a GH128 (subgroup I) endo-beta-1,3-glucanase (E102A mutant) from Amycolatopsis mediterranei (AmGH128_I) in complex with laminaritriose and laminaribiose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Glyco_hydro_cc domain-containing protein	polymer	263	27859.9	1	UniProt (G0FQ07) In PDB	Amycolatopsis mediterranei
2	B	beta-D-glucopyranose-(1-3)-beta-D-glucopyranose	branched		342.3	1	In PDB BIRD (PRD_900024) GlyTouCan (G36535HU)		beta-laminaribiose
3	C	beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose	branched		504.4	1	In PDB GlyTouCan (G00024MO)
4	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
5	A	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
6		water	water		18.0	258	Chemie (HOH)

Sequence modifications

A: 28 - 269 (UniProt: G0FQ07)

PDB	External Database	Details
Met 7	-	initiating methionine
Gly 8	-	expression tag
Ser 9	-	expression tag
Ser 10	-	expression tag
His 11	-	expression tag
His 12	-	expression tag
His 13	-	expression tag
His 14	-	expression tag
His 15	-	expression tag
His 16	-	expression tag
Ser 17	-	expression tag
Ser 18	-	expression tag
Gly 19	-	expression tag
Leu 20	-	expression tag
Val 21	-	expression tag
Pro 22	-	expression tag
Ala 23	-	expression tag
Gly 24	-	expression tag
Ser 25	-	expression tag
His 26	-	expression tag
Met 27	-	expression tag
Ala 102	Glu 102	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	27859.9
Branched	Number of molecules	2
	Total formula weight	846.7
Non-Polymers*	Number of molecules	2
	Total formula weight	171.5
All*	Total formula weight	28878.2

*Water molecules are not included.