6U44

Crystal structure of Methanoperedens nitroreducens elongation factor 2 H595N bound to GMPPCP and magnesium (monoclinic crystal form)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Elongation factor 2	polymer	753	83304.2	2	UniProt (A0A062V290) Pfam (PF00009) Pfam (PF03144) Pfam (PF14492) Pfam (PF03764) Pfam (PF00679)	Candidatus Methanoperedens nitroreducens	EF-2
2	C, J (A, B)	PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER	non-polymer		521.2	2	Chemie (GCP)
3	D, K (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	E, H, L, M (A, B)	CHLORIDE ION	non-polymer		35.5	4	Chemie (CL)
5	F, G, I, N, O... (A, B)	SULFATE ION	non-polymer		96.1	6	Chemie (SO4)
6	Q, R (A, B)	water	water		18.0	830	Chemie (HOH)

Sequence modifications

A, B: 1 - 729 (UniProt: A0A062V290)

PDB	External Database	Details
Met -23	-	initiating methionine
Gly -22	-	expression tag
Ser -21	-	expression tag
Asp -20	-	expression tag
Lys -19	-	expression tag
Ile -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
Gly -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag
Asn 595	His 595	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	166608.4
Non-Polymers*	Number of molecules	14
	Total formula weight	1809.2
All*	Total formula weight	168417.7

*Water molecules are not included.