6U28

Crystal structure of 1918 NS1-ED W187A in complex with the p85-beta-iSH2 domain of human PI3K

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, B)	Non-structural protein 1	polymer	145	16337.9	2	UniProt (Q99AU3) Pfam (PF00600)	Influenza A virus (strain A/Brevig Mission/1/1918 H1N1)	NS1,NS1A
2	B, D (C, D)	Phosphatidylinositol 3-kinase regulatory subunit beta	polymer	165	19893.4	2	UniProt (O00459) Pfam (PF16454)	Homo sapiens (Human)	PtdIns-3-kinase regulatory subunit beta,Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta,PtdIns-3-kinase regulatory subunit p85-beta

Sequence modifications

A, B: 86 - 230 (UniProt: Q99AU3)

PDB	External Database	Details
Ala 187	Trp 187	engineered mutation

C, D: 435 - 597 (UniProt: O00459)

PDB	External Database	Details
Gly 433	-	expression tag
Ser 434	-	expression tag
Ser 501	Cys 501	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	72462.6
All*	Total formula weight	72462.6

*Water molecules are not included.