6TTU
Ubiquitin Ligation to substrate by a cullin-RING E3 ligase at 3.7A resolution: NEDD8-CUL1-RBX1 N98R-SKP1-monomeric b-TRCP1dD-IkBa-UB~UBE2D2
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | T | F-box/WD repeat-containing protein 1A | polymer | 605 | 68960.8 | 1 | UniProt (Q9Y297) Pfam (PF00400) Pfam (PF12125) Pfam (PF12937) In PDB | Homo sapiens (Human) | E3RSIkappaB,Epididymis tissue protein Li 2a,F-box and WD repeats protein beta-TrCP,pIkappaBalpha-E3 receptor subunit |
| 2 | S | S-phase kinase-associated protein 1 | polymer | 163 | 18680.0 | 1 | UniProt (P63208) Pfam (PF03931) Pfam (PF01466) In PDB | Homo sapiens (Human) | Cyclin-A/CDK2-associated protein p19,p19A,Organ of Corti protein 2,OCP-2,Organ of Corti protein II,OCP-II,RNA polymerase II elongation factor-like protein,SIII,Transcription elongation factor B polypeptide 1-like,p19skp1 |
| 3 | C | Cullin-1 | polymer | 776 | 89800.4 | 1 | UniProt (Q13616) Pfam (PF00888) Pfam (PF10557) In PDB | Homo sapiens (Human) | CUL-1 |
| 4 | N | NEDD8 | polymer | 77 | 8661.1 | 1 | UniProt (Q15843) Pfam (PF00240) In PDB | Homo sapiens (Human) | Neddylin,Neural precursor cell expressed developmentally down-regulated protein 8,NEDD-8,Ubiquitin-like protein Nedd8 |
| 5 | R | E3 ubiquitin-protein ligase RBX1 | polymer | 108 | 12333.1 | 1 | UniProt (P62877) Pfam (PF12678) UniProt (by SIFTS) (H2QLR9) In PDB | Homo sapiens (Human) | E3 ubiquitin-protein transferase RBX1,Protein ZYP,RING finger protein 75,RING-box protein 1,Rbx1,Regulator of cullins 1,ROC1 |
| 6 | D | Ubiquitin-conjugating enzyme E2 D2 | polymer | 147 | 16745.1 | 1 | UniProt (P62837) Pfam (PF00179) In PDB | Homo sapiens (Human) | (E3-independent) E2 ubiquitin-conjugating enzyme D2,E2 ubiquitin-conjugating enzyme D2,Ubiquitin carrier protein D2,Ubiquitin-conjugating enzyme E2(17)KB 2,Ubiquitin-conjugating enzyme E2-17 kDa 2,Ubiquitin-protein ligase D2,p53-regulated ubiquitin-conjugating enzyme 1 |
| 7 | U | Polyubiquitin-C | polymer | 76 | 8576.8 | 1 | UniProt (P0CG48) Pfam (PF00240) In PDB | Homo sapiens (Human) | |
| 8 | I | CYS-LYS-LYS-ALA-ARG-HIS-ASP-SEP-GLY | polymer | 30 | 3782.8 | 1 | UniProt (by SIFTS) (P25963) In PDB | Homo sapiens | |
| 9 | R | ZINC ION | non-polymer | 65.4 | 3 | Chemie (ZN) |
Sequence modifications
N: 1 - 76 (UniProt: Q15843)
R: 1 - 108 (UniProt: P62877)
D: 1 - 147 (UniProt: P62837)
| PDB | External Database | Details |
|---|---|---|
| Ser 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Arg 98 | Asn 98 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ile 21 | Cys 21 | engineered mutation |
| Ala 107 | Cys 107 | engineered mutation |
| Asp 111 | Cys 111 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 227540.0 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 196.2 | |
| All* | Total formula weight | 227736.2 |
