6TTR

Crystal Structure of the coiled coil and GGDEF domain of DgcB from Caulobacter crescentus in complex with c-di-GMP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	GGDEF diguanylate cyclase DgcB	polymer	222	24000.7	2	UniProt (A0A0H3CAN8) Pfam (PF00990)	Caulobacter vibrioides (strain NA1000 / CB15N)
2	C, G (A, B)	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
3	D, E, F (A, B)	9,9'-[(2R,3R,3aS,5S,7aR,9R,10R,10aS,12S,14aR)-3,5,10,12-tetrahydroxy-5,12-dioxidooctahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-2,9-diyl]bis(2-amino-1,9-dihydro-6H-purin-6-one)	non-polymer		690.4	3	Chemie (C2E)

Sequence modifications

A, B: 153 - 353 (UniProt: A0A0H3CAN8)

PDB	External Database	Details
Met 132	-	initiating methionine
Gly 133	-	expression tag
Ser 134	-	expression tag
Ser 135	-	expression tag
His 136	-	expression tag
His 137	-	expression tag
His 138	-	expression tag
His 139	-	expression tag
His 140	-	expression tag
His 141	-	expression tag
Ser 142	-	expression tag
Ser 143	-	expression tag
Gly 144	-	expression tag
Leu 145	-	expression tag
Val 146	-	expression tag
Pro 147	-	expression tag
Arg 148	-	expression tag
Gly 149	-	expression tag
Ser 150	-	expression tag
His 151	-	expression tag
Met 152	-	expression tag
Ala 261	Glu 261	engineered mutation
Ala 262	Glu 262	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	48001.5
Non-Polymers*	Number of molecules	5
	Total formula weight	2261.2
All*	Total formula weight	50262.6

*Water molecules are not included.