6TMK

Cryo-EM structure of Toxoplasma gondii mitochondrial ATP synthase dimer, composite model

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, Y (q, Q)	ATPTG11	polymer	134	15425.4	2	UniProt (A0A125YPS4)	Toxoplasma gondii (strain ATCC 50853 / GT1)
2	B, Z (i, I)	ATPTG7	polymer	236	26866.8	2	UniProt (S7VXW3)	Toxoplasma gondii (strain ATCC 50853 / GT1)
3	AA, C (t, T)	ATPTG14	polymer	133	14424.5	2	UniProt (A0A125YLH9)	Toxoplasma gondii (strain ATCC 50853 / GT1)
4	BA, D (g, G)	ATPTG5	polymer	252	28162.6	2	UniProt (S7WD71)	Toxoplasma gondii (strain ATCC 50853 / GT1)
5	CA, E (o, O)	subunit k	polymer	157	17963.5	2	UniProt (A0A125YTD7)	Toxoplasma gondii (strain ATCC 50853 / GT1)
6	DA, F (k, K)	subunit a	polymer	224	24562.6	2	UniProt (A0A125YSI9)	Toxoplasma gondii (strain ATCC 50853 / GT1)
7	EA, G (j, J)	subunit i/j	polymer	229	26600.5	2	UniProt (S7UQ82)	Toxoplasma gondii (strain ATCC 50853 / GT1)
8	FA, H (s, S)	ATPTG13	polymer	128	14923.2	2	UniProt (A0A125YLN4)	Toxoplasma gondii (strain ATCC 50853 / GT1)
9	GA, I (u, U)	ATPTG15	polymer	126	13871.9	2	UniProt (A0A125YRP0)	Toxoplasma gondii (strain ATCC 50853 / GT1)
10	HA, J (h, H)	ATPTG6	polymer	239	27477.5	2	UniProt (A0A125YL08)	Toxoplasma gondii (strain ATCC 50853 / GT1)
11	IA, K (e, E)	ATPTG3	polymer	325	35159.2	2	UniProt (A0A125YLR0)	Toxoplasma gondii (strain ATCC 50853 / GT1)
12	JA, L (x, X)	ATPTG17	polymer	83	9486.7	2	UniProt (S7W180)	Toxoplasma gondii (strain ATCC 50853 / GT1)
13	KA, M (b, B)	subunit b	polymer	571	64811.6	2	UniProt (S7V2T0)	Toxoplasma gondii (strain ATCC 50853 / GT1)
14	LA, N (r, R)	ATPTG12	polymer	134	15400.6	2	UniProt (A0A125YKF7)	Toxoplasma gondii (strain ATCC 50853 / GT1)
15	MA, O (p, P)	ATPTG10	polymer	138	15165.2	2	UniProt (A0A125YMA7) Pfam (PF20716)	Toxoplasma gondii (strain ATCC 50853 / GT1)
16	NA, P (v, V)	subunit f	polymer	111	13030.0	2	UniProt (S7UQT7)	Toxoplasma gondii (strain ATCC 50853 / GT1)
17	OA, Q (l, L)	ATPTG8	polymer	208	23365.9	2	UniProt (S7W7F1)	Toxoplasma gondii (strain ATCC 50853 / GT1)
18	PA, R (c, C)	ATPTG1	polymer	398	46025.4	2	UniProt (S7W9J5) Pfam (PF02167)	Toxoplasma gondii (strain ATCC 50853 / GT1)
19	QA, S (d, D)	ATPTG2	polymer	310	35238.5	2	UniProt (A0A125YV76)	Toxoplasma gondii (strain ATCC 50853 / GT1)
20	RA, T (m, M)	subunit 8	polymer	205	22344.6	2	UniProt (A0A125YPQ4)	Toxoplasma gondii (strain ATCC 50853 / GT1)
21	SA, U (n, N)	ATPTG9	polymer	166	18426.8	2	UniProt (A0A125YUZ2)	Toxoplasma gondii (strain ATCC 50853 / GT1)
22	TA, V (f, F)	ATPTG4	polymer	267	28557.4	2	UniProt (S7UVZ7) Pfam (PF00085)	Toxoplasma gondii (strain ATCC 50853 / GT1)
23	UA, W (w, W)	ATPTG16	polymer	106	11587.4	2	UniProt (S7VTI0)	Toxoplasma gondii (strain ATCC 50853 / GT1)
24	VA, X (a, A)	subunit d	polymer	536	61362.3	2	UniProt (S7V493)	Toxoplasma gondii (strain ATCC 50853 / GT1)
25	RB, WA (i2, i1)	Inhibitor of F1	polymer	145	16167.5	2	UniProt (A0A125YJP2)	Toxoplasma gondii (strain ATCC 50853 / GT1)
26	SB, TB, UB, XA, YA... (A2, E2, C2, A1, E1...)	ATP synthase subunit alpha,subunit alpha	polymer	565	61189.2	6	UniProt (S7UU80) Pfam (PF02874) Pfam (PF00006) Pfam (PF00306)	Toxoplasma gondii (strain ATCC 50853 / GT1)
27	AB, BB, CB, VB, WB... (B2, F2, D2, B1, F1...)	ATP synthase subunit beta	polymer	560	59983.3	6	UniProt (A0A125YYY4) Pfam (PF02874) Pfam (PF00006) Pfam (PF22919)	Toxoplasma gondii (strain ATCC 50853 / GT1)
28	DB, YB (g2, g1)	ATP synthase subunit gamma	polymer	314	34573.0	2	UniProt (A0A125YUH0) Pfam (PF00231)	Toxoplasma gondii (strain ATCC 50853 / GT1)
29	EB, ZB (d2, d1)	ATP synthase subunit delta	polymer	183	19476.1	2	UniProt (A0A125YRE2) Pfam (PF02823)	Toxoplasma gondii (strain ATCC 50853 / GT1)
30	AC, FB (e2, e1)	ATP synthase subunit epsilon	polymer	73	8492.7	2	UniProt (S7VV10) Pfam (PF04627)	Toxoplasma gondii (strain ATCC 50853 / GT1)
31	BC, GB (G2, G1)	Oligomycin sensitivity conferring protein (OSCP)	polymer	252	27670.0	2	UniProt (A0A125YKF8) Pfam (PF00213)	Toxoplasma gondii (strain ATCC 50853 / GT1)
32	CC, DC, EC, FC, GC... (H2, I2, J2, K2, L2...)	subunit c	polymer	166	17753.5	20	UniProt (A0A125YJV2) Pfam (PF00137)	Toxoplasma gondii (strain ATCC 50853 / GT1)
33	ID, LD, MC, PC, VD... (O, o, V, v)	1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE	non-polymer		790.1	6	Chemie (PC1)
34	AE, BE, CD, DE, ED... (C, D, c, d, O...)	DODECYL-BETA-D-MALTOSIDE	non-polymer		510.6	14	Chemie (LMT)
35	AD, BD, CE, GD, KD... (v, c, D, d, O...)	CARDIOLIPIN	non-polymer		1464.0	18	Chemie (CDL)
36	DD, MD, ND, QC, RC... (c, J, j, C)	1,2-dioleoyl-sn-glycero-3-phosphoethanolamine	non-polymer		744.0	6	Chemie (PEE)
37	EE, FE, GE, JE, KE... (A2, E2, C2, A1, E1...)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	6	Chemie (ATP)
38	HE, IE, ME, NE (B2, D2, B1, D1)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	4	Chemie (ADP)

Sequence modifications

g, G: 1 - 252 (UniProt: S7WD71)

PDB	External Database	Details
Val 51	Phe 51	conflict
Cys 73	Ser 73	conflict
Lys 110	Glu 110	conflict
Thr 233	Met 233	conflict

h, H: 1 - 239 (UniProt: A0A125YL08)

PDB	External Database	Details
Asn 89	His 89	conflict

e, E: 1 - 325 (UniProt: A0A125YLR0)

PDB	External Database	Details
-	Lys 14	deletion
Pro 63	Ser 64	conflict
Leu 99	Pro 100	conflict
Ala 312	Thr 313	conflict

x, X: 1 - 76 (UniProt: S7W180)

PDB	External Database	Details
Trp 77	-	expression tag
Met 78	-	expression tag
Phe 79	-	expression tag
Gly 80	-	expression tag
Asn 81	-	expression tag
Ser 82	-	expression tag
Tyr 83	-	expression tag

b, B: 1 - 571 (UniProt: S7V2T0)

PDB	External Database	Details
Leu 48	Ser 48	conflict
Thr 472	Ala 472	conflict

v, V: 1 - 111 (UniProt: S7UQT7)

PDB	External Database	Details
Ala 54	Val 54	conflict

n, N: 1 - 166 (UniProt: A0A125YUZ2)

PDB	External Database	Details
Ser 140	Ala 140	conflict

a, A: 1 - 536 (UniProt: S7V493)

PDB	External Database	Details
Thr 351	Ala 484	conflict

A2, E2, C2, A1, E1, C1: 1 - 538 (UniProt: S7UU80)
A2, E2, C2, A1, E1, C1: 539 - 565 (PDB: 6TMK)

Contents of the asymmetric unit

Polymers	Number of chains	90
	Total formula weight	2515343.9
Non-Polymers*	Number of molecules	54
	Total formula weight	47458.3
All*	Total formula weight	2562802.2

*Water molecules are not included.