6TIS

DROSOPHILA GDP-TUBULIN

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Tubulin alpha-1 chain	polymer	450	49989.3	2	UniProt (P06603) Pfam (PF00091) Pfam (PF03953)	Drosophila melanogaster (Fruit fly)
2	B, D (B, D)	Tubulin beta-1 chain	polymer	447	50194.1	2	UniProt (Q24560) Pfam (PF00091) Pfam (PF03953)	Drosophila melanogaster (Fruit fly)	Beta-1-tubulin
3	E (E)	Stathmin-4	polymer	143	16851.1	1	UniProt (P63043) Pfam (PF00836)	Rattus norvegicus (Norway rat)	Stathmin-like protein B3,RB3
4	F, R (A, C)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	2	Chemie (GTP)
5	G, S (A, C)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6	H, I, J, K, N... (A, B, C, D)	SULFATE ION	non-polymer		96.1	11	Chemie (SO4)
7	L, Q (A, B)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
8	M, U (B, D)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	2	Chemie (GDP)
9	AA, BA, CA, Y, Z (C, D, E, A, B)	water	water		18.0	548	Chemie (HOH)

Sequence modifications

A, C: 1 - 450 (UniProt: P06603)

PDB	External Database	Details
Arg 40	Lys 40	engineered mutation

E: 4 - 145 (UniProt: P63043)

PDB	External Database	Details
Met 3	-	initiating methionine
Ala 4	Ser 48	engineered mutation
Ala 14	Cys 58	engineered mutation
Trp 20	Phe 64	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	217217.9
Non-Polymers*	Number of molecules	19
	Total formula weight	3222.3
All*	Total formula weight	220440.2

*Water molecules are not included.