6TIP
Engineered streptavidin variant (YNAFM) in complex with the Strep-tag II peptide
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Streptavidin | polymer | 127 | 13438.6 | 2 | UniProt (P22629) Pfam (PF01382) | Streptomyces avidinii | |
| 2 | C, D (P, Q) | Strep-tag II peptide | polymer | 10 | 1218.3 | 2 | synthetic construct | ||
| 3 | E (P) | AMINO GROUP | non-polymer | 16.0 | 1 | Chemie (NH2) | |||
| 4 | F, G (A, P) | water | water | 18.0 | 52 | Chemie (HOH) |
Sequence modifications
A, B: 14 - 139 (UniProt: P22629)
| PDB | External Database | Details |
|---|---|---|
| Met 13 | - | initiating methionine |
| Val 44 | Glu 68 | engineered mutation |
| Thr 45 | Ser 69 | engineered mutation |
| Arg 47 | Val 71 | engineered mutation |
| Tyr 117 | Ala 141 | engineered mutation |
| Phe 120 | Trp 144 | engineered mutation |
| Met 121 | Lys 145 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 29313.9 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 16.0 | |
| All* | Total formula weight | 29329.9 |
