6T6M

Y201W mutant of the orange carotenoid protein from Synechocystis at pH 5.5

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Orange carotenoid-binding protein	polymer	332	36454.5	1	UniProt (P74102) Pfam (PF09150) Pfam (PF02136)	Synechocystis sp. PCC 6803	OCP
2	B (A)	beta,beta-caroten-4-one	non-polymer		550.9	1	Chemie (ECH)
3	C (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
4	D (A)	HISTIDINE	non-polymer		156.2	1	Chemie (HIS)
5	E (A)	water	water		18.0	337	Chemie (HOH)

Sequence modifications

A: 1 - 317 (UniProt: P74102)

PDB	External Database	Details
Met -14	-	initiating methionine
Arg -13	-	expression tag
Gly -12	-	expression tag
Ser -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
Thr -4	-	expression tag
Asp -3	-	expression tag
Pro -2	-	expression tag
Ala -1	-	expression tag
Thr 0	-	expression tag
Trp 201	Tyr 201	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	36454.5
Non-Polymers*	Number of molecules	3
	Total formula weight	799.1
All*	Total formula weight	37253.6

*Water molecules are not included.