6T0F
Crystal structure of CYP124 in complex with cholest-4-en-3-one
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Methyl-branched lipid omega-hydroxylase | polymer | 435 | 48869.0 | 4 | UniProt (P9WPP3) Pfam (PF00067) | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) | Cholest-4-en-3-one C26-monooxygenase,Cholest-4-en-3-one C26-monooxygenase [(25R)-3-oxocholest-4-en-26-oate forming],Cholesterol C26-monooxygenase,Cholesterol C26-monooxygenase [(25R)-3beta-hydroxycholest-5-en-26-oate forming],Cytochrome P450 124,Steroid C26-monooxygenase,Steroid C27-monooxygenase |
| 2 | CA, E, N, V (D, A, B, C) | PROTOPORPHYRIN IX CONTAINING FE | non-polymer | 616.5 | 4 | Chemie (HEM) | |||
| 3 | DA, F, O, W (D, A, B, C) | (8ALPHA,9BETA)-CHOLEST-4-EN-3-ONE | non-polymer | 384.6 | 4 | Chemie (K2B) | |||
| 4 | EA, G, P, X (D, A, B, C) | 3[N-MORPHOLINO]PROPANE SULFONIC ACID | non-polymer | 209.3 | 4 | Chemie (MPO) | |||
| 5 | GA, H, HA, I, J... (D, A, B, C) | GLYCEROL | non-polymer | 92.1 | 12 | Chemie (GOL) | |||
| 6 | AA, BA, FA, L, T... (C, D, A, B) | TRIETHYLENE GLYCOL | non-polymer | 150.2 | 6 | Chemie (PGE) | |||
| 7 | IA, JA, KA, LA (A, B, C, D) | water | water | 18.0 | 2591 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 428 (UniProt: P9WPP3)
| PDB | External Database | Details |
|---|---|---|
| Met -6 | - | initiating methionine |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| His -1 | - | expression tag |
| His 0 | - | expression tag |
| Thr 65 | Ala 65 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 195475.8 | |
| Non-Polymers* | Number of molecules | 30 |
| Total formula weight | 6847.7 | |
| All* | Total formula weight | 202323.5 |
