6T0E
The glucuronoyl esterase OtCE15A S267A variant from Opitutus terrae in complex with benzyl D-glucuronoate and D-glucuronate
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | glucuronoyl esterase OtCE15A | polymer | 421 | 46132.5 | 2 | UniProt (B1ZMF4) Pfam (PF22244) | Opitutus terrae (strain DSM 11246 / JCM 15787 / PB90-1) | |
| 2 | C, NA (A, B) | alpha-D-glucopyranuronic acid | non-polymer | 194.1 | 2 | Chemie (GCU) | |||
| 3 | D (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) PubChem (312) | |||
| 4 | E (A) | benzyl alpha-D-glucopyranuronate | non-polymer | 284.3 | 1 | Chemie (M55) PubChem (14602686) PubChem (14602688) PubChem (29973356) PubChem (29973359) PubChem (29973362) PubChem (45038287) PubChem (177610380) PubChem (29973365) PubChem (68629822) PubChem (124934016) PubChem (58148857) PubChem (58372797) PubChem (167624771) PubChem (68521378) PubChem (97302335) PubChem (124546138) PubChem (124584154) PubChem (126968585) PubChem (132935950) PubChem (142535561) PubChem (156621579) PubChem (156621593) PubChem (159705855) PubChem (159705856) PubChem (163285259) PubChem (165162702) PubChem (173921037) | |||
| 5 | F (A) | TETRAETHYLENE GLYCOL | non-polymer | 194.2 | 1 | Chemie (PG4) PubChem (8200) | |||
| 6 | AB, BB, CB, G, H... (B, A) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 34 | Chemie (EDO) | |||
| 7 | AA, BA, CA, DB, EB... (A, B) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 6 | Chemie (PEG) PubChem (8117) | |||
| 8 | DA, EA, FA, FB, GA (A, B) | TRIETHYLENE GLYCOL | non-polymer | 150.2 | 5 | Chemie (PGE) | |||
| 9 | HA (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) PubChem (888) | |||
| 10 | GB, HB, IA, IB, JA... (B, A) | trimethylamine oxide | non-polymer | 75.1 | 7 | Chemie (TMO) PubChem (1145) | |||
| 11 | KB, LA, LB, MA, MB (B, A) | DIMETHYL SULFOXIDE | non-polymer | 78.1 | 5 | Chemie (DMS) PubChem (679) | |||
| 12 | NB, OB (A, B) | water | water | 18.0 | 412 | Chemie (HOH) |
Sequence modifications
A, B: 33 - 432 (UniProt: B1ZMF4)
| PDB | External Database | Details |
|---|---|---|
| Met 12 | - | initiating methionine |
| Gly 13 | - | expression tag |
| Ser 14 | - | expression tag |
| Ser 15 | - | expression tag |
| His 16 | - | expression tag |
| His 17 | - | expression tag |
| His 18 | - | expression tag |
| His 19 | - | expression tag |
| His 20 | - | expression tag |
| His 21 | - | expression tag |
| Ser 22 | - | expression tag |
| Ser 23 | - | expression tag |
| Glu 24 | - | expression tag |
| Asn 25 | - | expression tag |
| Leu 26 | - | expression tag |
| Tyr 27 | - | expression tag |
| Phe 28 | - | expression tag |
| Gln 29 | - | expression tag |
| Gly 30 | - | expression tag |
| His 31 | - | expression tag |
| Ser 32 | - | expression tag |
| Ala 267 | Ser 267 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 92265.1 | |
| Non-Polymers* | Number of molecules | 63 |
| Total formula weight | 5340.9 | |
| All* | Total formula weight | 97605.9 |
