6T0E
The glucuronoyl esterase OtCE15A S267A variant from Opitutus terrae in complex with benzyl D-glucuronoate and D-glucuronate
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | glucuronoyl esterase OtCE15A | polymer | 421 | 46132.5 | 2 | UniProt (B1ZMF4) Pfam (PF22244) | Opitutus terrae (strain DSM 11246 / JCM 15787 / PB90-1) | |
| 2 | C, NA (A, B) | alpha-D-glucopyranuronic acid | non-polymer | 194.1 | 2 | Chemie (GCU) | |||
| 3 | D (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 4 | E (A) | benzyl alpha-D-glucopyranuronate | non-polymer | 284.3 | 1 | Chemie (M55) | |||
| 5 | F (A) | TETRAETHYLENE GLYCOL | non-polymer | 194.2 | 1 | Chemie (PG4) | |||
| 6 | AB, BB, CB, G, H... (B, A) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 34 | Chemie (EDO) | |||
| 7 | AA, BA, CA, DB, EB... (A, B) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 6 | Chemie (PEG) | |||
| 8 | DA, EA, FA, FB, GA (A, B) | TRIETHYLENE GLYCOL | non-polymer | 150.2 | 5 | Chemie (PGE) | |||
| 9 | HA (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 10 | GB, HB, IA, IB, JA... (B, A) | trimethylamine oxide | non-polymer | 75.1 | 7 | Chemie (TMO) | |||
| 11 | KB, LA, LB, MA, MB (B, A) | DIMETHYL SULFOXIDE | non-polymer | 78.1 | 5 | Chemie (DMS) | |||
| 12 | NB, OB (A, B) | water | water | 18.0 | 412 | Chemie (HOH) |
Sequence modifications
A, B: 33 - 432 (UniProt: B1ZMF4)
| PDB | External Database | Details |
|---|---|---|
| Met 12 | - | initiating methionine |
| Gly 13 | - | expression tag |
| Ser 14 | - | expression tag |
| Ser 15 | - | expression tag |
| His 16 | - | expression tag |
| His 17 | - | expression tag |
| His 18 | - | expression tag |
| His 19 | - | expression tag |
| His 20 | - | expression tag |
| His 21 | - | expression tag |
| Ser 22 | - | expression tag |
| Ser 23 | - | expression tag |
| Glu 24 | - | expression tag |
| Asn 25 | - | expression tag |
| Leu 26 | - | expression tag |
| Tyr 27 | - | expression tag |
| Phe 28 | - | expression tag |
| Gln 29 | - | expression tag |
| Gly 30 | - | expression tag |
| His 31 | - | expression tag |
| Ser 32 | - | expression tag |
| Ala 267 | Ser 267 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 92265.1 | |
| Non-Polymers* | Number of molecules | 63 |
| Total formula weight | 5340.9 | |
| All* | Total formula weight | 97605.9 |
