6SZ4

The glucuronoyl esterase OtCE15A H408A variant from Opitutus terrae in complex with, and covalently linked to, D-glucuronate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	glucuronoyl esterase OtCE15A	polymer	421	46081.5	1	UniProt (B1ZMF4) Pfam (PF22244)	Opitutus terrae PB90-1
2	B (A)	beta-D-glucopyranuronic acid	non-polymer		194.1	1	Chemie (BDP)
3	C (A)	FORMIC ACID	non-polymer		46.0	1	Chemie (FMT)
4	D (A)	water	water		18.0	109	Chemie (HOH)

Sequence modifications

A: 33 - 432 (UniProt: B1ZMF4)

PDB	External Database	Details
Met 12	-	initiating methionine
Gly 13	-	expression tag
Ser 14	-	expression tag
Ser 15	-	expression tag
His 16	-	expression tag
His 17	-	expression tag
His 18	-	expression tag
His 19	-	expression tag
His 20	-	expression tag
His 21	-	expression tag
Ser 22	-	expression tag
Ser 23	-	expression tag
Glu 24	-	expression tag
Asn 25	-	expression tag
Leu 26	-	expression tag
Tyr 27	-	expression tag
Phe 28	-	expression tag
Gln 29	-	expression tag
Gly 30	-	expression tag
His 31	-	expression tag
Ser 32	-	expression tag
Ala 408	His 408	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	46081.5
Non-Polymers*	Number of molecules	2
	Total formula weight	240.2
All*	Total formula weight	46321.6

*Water molecules are not included.