6SYX
Hydrogenase-2 variant R479K - reduced sample exposed to pure oxygen
This is a non-PDB format compatible entry.
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | SSS, TTT | Hydrogenase-2 small chain | polymer | 298 | 32371.5 | 2 | UniProt (P69741) Pfam (PF01058) Pfam (PF14720) In PDB | Escherichia coli K-12 | HYD2,Membrane-bound hydrogenase 2 small subunit,NiFe hydrogenase |
2 | LLL, MMM | Hydrogenase-2 large chain | polymer | 567 | 62529.9 | 2 | UniProt (V0V766) In PDB | Escherichia coli 908519 | |
3 | SSS, TTT | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 4 | Chemie (SF4) | |||
4 | SSS, TTT | FE3-S4 CLUSTER | non-polymer | 295.8 | 2 | Chemie (F3S) | |||
5 | MMM, SSS, LLL, TTT | GLYCEROL | non-polymer | 92.1 | 7 | Chemie (GOL) | |||
6 | LLL, MMM | CARBONMONOXIDE-(DICYANO) IRON | non-polymer | 135.9 | 2 | Chemie (FCO) | |||
7 | LLL, MMM | NICKEL (II) ION | non-polymer | 58.7 | 2 | Chemie (NI) | |||
8 | LLL, TTT, MMM | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) | |||
9 | MMM, LLL | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) | |||
10 | MMM, LLL | OXYGEN MOLECULE | non-polymer | 32.0 | 2 | Chemie (OXY) | |||
11 | water | water | 18.0 | 1809 | Chemie (HOH) |
Sequence modifications
SSS, TTT: -1 - 290 (UniProt: P69741)
LLL, MMM: 1 - 567 (UniProt: V0V766)
PDB | External Database | Details |
---|---|---|
His 291 | - | expression tag |
His 292 | - | expression tag |
His 293 | - | expression tag |
His 294 | - | expression tag |
His 295 | - | expression tag |
His 296 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Lys 479 | Arg 479 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 189802.8 | |
Non-Polymers* | Number of molecules | 25 |
Total formula weight | 3264.1 | |
All* | Total formula weight | 193066.9 |