6SYV

The glucuronoyl esterase OtCE15A S267A variant from Opitutus terrae in complex with D-glucuronate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	glucuronoyl esterase OtCE15A	polymer	421	46132.5	1	UniProt (B1ZMF4) In PDB	Opitutus terrae PB90-1
2	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
3	A	1,2-ETHANEDIOL	non-polymer		62.1	11	Chemie (EDO)
4	A	alpha-D-glucopyranuronic acid	non-polymer		194.1	1	Chemie (GCU)
5	A	beta-D-glucopyranuronic acid	non-polymer		194.1	1	Chemie (BDP)
6	A	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	3	Chemie (PEG)
7		water	water		18.0	368	Chemie (HOH)

Sequence modifications

A: 33 - 432 (UniProt: B1ZMF4)

PDB	External Database	Details
Met 12	-	initiating methionine
Gly 13	-	expression tag
Ser 14	-	expression tag
Ser 15	-	expression tag
His 16	-	expression tag
His 17	-	expression tag
His 18	-	expression tag
His 19	-	expression tag
His 20	-	expression tag
His 21	-	expression tag
Ser 22	-	expression tag
Ser 23	-	expression tag
Glu 24	-	expression tag
Asn 25	-	expression tag
Leu 26	-	expression tag
Tyr 27	-	expression tag
Phe 28	-	expression tag
Gln 29	-	expression tag
Gly 30	-	expression tag
His 31	-	expression tag
Ser 32	-	expression tag
Ala 267	Ser 267	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	46132.5
Non-Polymers*	Number of molecules	17
	Total formula weight	1413.7
All*	Total formula weight	47546.2

*Water molecules are not included.