6SXR
E221Q mutant of GH54 a-l-arabinofuranosidase soaked with 4-nitrophenyl a-l-arabinofuranoside
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | Alpha-L-arabinofuranosidase B | polymer | 482 | 50882.6 | 1 | UniProt (Q8NK89) Pfam (PF09206) Pfam (PF05270) In PDB | Aspergillus kawachii IFO 4308 | Arabinosidase B |
| 2 | B | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 1 | In PDB GlyTouCan (G42666HT) | |||
| 3 | A | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 1 | Chemie (NAG) | |||
| 4 | A | SULFATE ION | non-polymer | 96.1 | 3 | Chemie (SO4) | |||
| 5 | A | ACETATE ION | non-polymer | 59.0 | 2 | Chemie (ACT) | |||
| 6 | A | 4-nitrophenyl alpha-L-arabinofuranoside | non-polymer | 271.2 | 3 | Chemie (KHP) | |||
| 7 | A | 1,2-ETHANEDIOL | non-polymer | 62.1 | 13 | Chemie (EDO) | |||
| 8 | A | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 8 | Chemie (PEG) | |||
| 9 | A | TRIETHYLENE GLYCOL | non-polymer | 150.2 | 1 | Chemie (PGE) | |||
| 10 | A | PENTAETHYLENE GLYCOL | non-polymer | 238.3 | 2 | Chemie (1PE) | |||
| 11 | water | water | 18.0 | 352 | Chemie (HOH) |
Sequence modifications
A: 19 - 499 (UniProt: Q8NK89)
| PDB | External Database | Details |
|---|---|---|
| Met 18 | - | initiating methionine |
| Gln 221 | Glu 221 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 50882.6 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 424.4 | |
| Non-Polymers* | Number of molecules | 33 |
| Total formula weight | 3723.7 | |
| All* | Total formula weight | 55030.7 |
