6SP8
Structure of hyperstable haloalkane dehalogenase variant DhaA115 prepared by the 'soak-and-freeze' method under 150 bar of krypton pressure
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Haloalkane dehalogenase | polymer | 291 | 33400.2 | 2 | UniProt (P0A3G2) Pfam (PF00561) In PDB | Rhodococcus rhodochrous | |
2 | B, A | KRYPTON | non-polymer | 83.8 | 21 | Chemie (KR) | |||
3 | B, A | THIOCYANATE ION | non-polymer | 58.1 | 6 | Chemie (SCN) | |||
4 | B, A | 2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 282.3 | 2 | Chemie (B3P) | |||
5 | B, A | GLYCEROL | non-polymer | 92.1 | 8 | Chemie (GOL) | |||
6 | water | water | 18.0 | 617 | Chemie (HOH) |
Sequence modifications
A, B: 3 - 293 (UniProt: P0A3G2)
PDB | External Database | Details |
---|---|---|
Ser 20 | Glu 20 | engineered mutation |
Arg 80 | Phe 80 | engineered mutation |
Phe 128 | Cys 128 | engineered mutation |
Leu 148 | Thr 148 | engineered mutation |
Pro 155 | Ala 155 | engineered mutation |
Ile 172 | Ala 172 | engineered mutation |
Phe 176 | Cys 176 | engineered mutation |
Trp 198 | Asp 198 | engineered mutation |
Trp 219 | Val 219 | engineered mutation |
Leu 262 | Cys 262 | engineered mutation |
Phe 266 | Asp 266 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 66800.5 | |
Non-Polymers* | Number of molecules | 37 |
Total formula weight | 3409.7 | |
All* | Total formula weight | 70210.2 |