6SOS
Engineered streptavidin variant (ENAGY) in complex with the Twin-Strep-tag peptide
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Streptavidin | polymer | 127 | 13346.4 | 4 | UniProt (P22629) Pfam (PF01382) | Streptomyces avidinii | |
| 2 | E, F (P, R) | Twin-Strep-tag peptide | polymer | 32 | 3166.3 | 2 | synthetic construct | ||
| 3 | G, H, I, J, K (A, B, C, D, P) | water | water | 18.0 | 129 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 14 - 139 (UniProt: P22629)
| PDB | External Database | Details |
|---|---|---|
| Met 13 | - | initiating methionine |
| Val 44 | Glu 68 | engineered mutation |
| Thr 45 | Ser 69 | engineered mutation |
| Arg 47 | Val 71 | engineered mutation |
| Glu 117 | Ala 141 | engineered mutation |
| Gly 120 | Trp 144 | engineered mutation |
| Tyr 121 | Lys 145 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 59718.4 | |
| All* | Total formula weight | 59718.4 |
