6SOS

Engineered streptavidin variant (ENAGY) in complex with the Twin-Strep-tag peptide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Streptavidin	polymer	127	13346.4	4	UniProt (P22629) Pfam (PF01382) In PDB	Streptomyces avidinii
2	P, R	Twin-Strep-tag peptide	polymer	32	3166.3	2		synthetic construct
3		water	water		18.0	129	Chemie (HOH)

Sequence modifications

A, B, C, D: 14 - 139 (UniProt: P22629)

PDB	External Database	Details
Met 13	-	initiating methionine
Val 44	Glu 68	engineered mutation
Thr 45	Ser 69	engineered mutation
Arg 47	Val 71	engineered mutation
Glu 117	Ala 141	engineered mutation
Gly 120	Trp 144	engineered mutation
Tyr 121	Lys 145	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	59718.4
All*	Total formula weight	59718.4

*Water molecules are not included.