6SOK
Engineered streptavidin variant (VTAR) in complex with the Twin-Strep-tag peptide
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Streptavidin | polymer | 127 | 13383.6 | 4 | UniProt (P22629) Pfam (PF01382) | Streptomyces avidinii | |
| 2 | E, F, G, H (P, Q, R, S) | Twin-Strep-tag peptide | polymer | 32 | 3166.3 | 4 | synthetic construct | ||
| 3 | I, J, K, L (P, Q, R, S) | AMINO GROUP | non-polymer | 16.0 | 4 | Chemie (NH2) | |||
| 4 | M, N, O, P, Q... (A, B, C, D, P...) | water | water | 18.0 | 242 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 14 - 139 (UniProt: P22629)
| PDB | External Database | Details |
|---|---|---|
| Met 13 | - | initiating methionine |
| Val 44 | Glu 68 | engineered mutation |
| Thr 45 | Ser 69 | engineered mutation |
| Arg 47 | Val 71 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 66199.5 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 64.1 | |
| All* | Total formula weight | 66263.6 |
