6SNH

Cryo-EM structure of yeast ALG6 in complex with 6AG9 Fab and Dol25-P-Glc

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	X	Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase	polymer	562	64423.2	1	UniProt (Q12001) Pfam (PF03155) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Asparagine-linked glycosylation protein 6,Dol-P-Glc:Man(9)GlcNAc(2)-PP-Dol alpha-1,3-glucosyltransferase,Dolichyl-P-Glc:Man9GlcNAc2-PP-dolichyl glucosyltransferase
2	H	6AG9 Fab heavy chain	polymer	234	24953.7	1		synthetic construct
3	L	6AG9 Fab light chain	polymer	217	23650.3	1		synthetic construct
4	X	glucosyl-dolichol phosphate	non-polymer		602.7	1	Chemie (LMH)
5		water	water		18.0	1	Chemie (HOH)

Sequence modifications

X: 1 - 544 (UniProt: Q12001)

PDB	External Database	Details
Gly -17	-	expression tag
Pro -16	-	expression tag
Gly -15	-	expression tag
Gly -14	-	expression tag
Ser -13	-	expression tag
Thr -12	-	expression tag
Gly -11	-	expression tag
Arg -10	-	expression tag
Leu -9	-	expression tag
Ala -8	-	expression tag
Gly -7	-	expression tag
Ala -6	-	expression tag
Gly -5	-	expression tag
Gly -4	-	expression tag
Glu -3	-	expression tag
Phe -2	-	expression tag
Val -1	-	expression tag
Asp 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	113027.1
Non-Polymers*	Number of molecules	1
	Total formula weight	602.7
All*	Total formula weight	113629.9

*Water molecules are not included.