6SJG
Cryo-EM structure of the RecBCD no Chi negative control complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | B | RecBCD enzyme subunit RecB | polymer | 1181 | 134123.7 | 1 | UniProt (P08394) Pfam (PF00580) Pfam (PF13361) Pfam (PF12705) In PDB | Escherichia coli | Exodeoxyribonuclease V 135 kDa polypeptide,Exodeoxyribonuclease V beta chain,Exonuclease V subunit RecB,ExoV subunit RecB,Helicase/nuclease RecBCD subunit RecB |
| 2 | C | RecBCD enzyme subunit RecC | polymer | 1122 | 128974.1 | 1 | UniProt (P07648) Pfam (PF04257) Pfam (PF17946) In PDB | Escherichia coli | Exodeoxyribonuclease V 125 kDa polypeptide,Exodeoxyribonuclease V gamma chain,Exonuclease V subunit RecC,ExoV subunit RecC |
| 3 | D | RecBCD enzyme subunit RecD | polymer | 608 | 66990.4 | 1 | UniProt (P04993) Pfam (PF21185) Pfam (PF13245) Pfam (PF13538) In PDB | Escherichia coli | Exodeoxyribonuclease V 67 kDa polypeptide,Exodeoxyribonuclease V alpha chain,Exonuclease V subunit RecD,ExoV subunit RecD,Helicase/nuclease RecBCD subunit RecD |
| 4 | X | Forked DNA substrate | polymer | 85 | 26049.6 | 1 | synthetic construct |
Sequence modifications
B: 1 - 1180 (UniProt: P08394)
| PDB | External Database | Details |
|---|---|---|
| Gly 0 | - | expression tag |
| Ala 1080 | Asp 1080 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 356137.8 | |
| All* | Total formula weight | 356137.8 |
