6SIC
Cryo-EM structure of the Type III-B Cmr-beta bound to cognate target RNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C (A, B, C) | CRISPR-associated protein, Cmr5 family | polymer | 155 | 17961.8 | 3 | UniProt (F0NDX5) | Sulfolobus islandicus REY15A | |
| 2 | D, E, F, G (D, E, F, G) | CRISPR-associated RAMP protein, Cmr4 family | polymer | 286 | 32322.4 | 4 | UniProt (F0NDX6) Pfam (PF03787) | Sulfolobus islandicus REY15A | |
| 3 | H (H) | CRISPR-associated protein, Cmr3 family | polymer | 313 | 36008.5 | 1 | UniProt (F0NDX1) Pfam (PF09700) | Sulfolobus islandicus REY15A | |
| 4 | I (I) | CRISPR-associated RAMP protein, Cmr6 family | polymer | 296 | 33945.9 | 1 | UniProt (F0NDX3) Pfam (PF03787) | Sulfolobus islandicus REY15A | |
| 5 | J (J) | CRISPR-associated RAMP protein, Cmr1 family | polymer | 476 | 55389.0 | 1 | UniProt (F0NDX4) Pfam (PF03787) | Sulfolobus islandicus REY15A | |
| 6 | K (K) | CRISPR-associated protein, Cmr2 family | polymer | 1037 | 120856.5 | 1 | UniProt (F0NDX2) Pfam (PF12469) Pfam (PF22335) | Sulfolobus islandicus REY15A | |
| 7 | AA, BA, CA, DA, EA... (L, M, N, O, P...) | CRISPR-associated protein Cmrx | polymer | 174 | 19705.6 | 22 | UniProt (F0NDX7) Pfam (PF19021) | Sulfolobus islandicus REY15A | |
| 8 | FA (U) | Cognate target RNA | polymer | 46 | 14704.7 | 1 | Sulfolobus islandicus REY15A | ||
| 9 | GA (V) | crRNA | polymer | 51 | 16409.9 | 1 | GenBank (323473489) | Sulfolobus islandicus REY15A | |
| 10 | JA (K) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) |
Sequence modifications
D, E, F, G: 1 - 286 (UniProt: F0NDX6)
I: 1 - 283 (UniProt: F0NDX3)
J: 6 - 476 (UniProt: F0NDX4)
V: 1 - 51 (GenBank: 323473489)
| PDB | External Database | Details |
|---|---|---|
| Ala 31 | Asp 31 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ala 284 | - | expression tag |
| Ala 285 | - | expression tag |
| Ala 286 | - | expression tag |
| His 287 | - | expression tag |
| His 288 | - | expression tag |
| His 289 | - | expression tag |
| His 290 | - | expression tag |
| His 291 | - | expression tag |
| His 292 | - | expression tag |
| His 293 | - | expression tag |
| His 294 | - | expression tag |
| His 295 | - | expression tag |
| His 296 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Glu 2 | - | expression tag |
| Glu 3 | - | expression tag |
| Leu 4 | - | expression tag |
| Leu 5 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| A 1 | C 2068518 | conflict |
| U 3 | G 2068520 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 35 |
| Total formula weight | 894012.8 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 65.4 | |
| All* | Total formula weight | 894078.2 |
