6SH8
Cryo-EM structure of the Type III-B Cmr-beta bound to cognate target RNA and AMPPnP, state 2, in the presence of ssDNA
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C | CRISPR-associated protein, Cmr5 family | polymer | 155 | 17961.8 | 3 | UniProt (F0NDX5) In PDB | Sulfolobus islandicus REY15A | |
2 | D, E, F, G | CRISPR-associated RAMP protein, Cmr4 family | polymer | 286 | 32322.4 | 4 | UniProt (F0NDX6) Pfam (PF03787) In PDB | Sulfolobus islandicus REY15A | |
3 | H | CRISPR-associated protein, Cmr3 family | polymer | 313 | 36008.5 | 1 | UniProt (F0NDX1) Pfam (PF09700) In PDB | Sulfolobus islandicus REY15A | |
4 | I | CRISPR-associated RAMP protein, Cmr6 family | polymer | 296 | 33945.9 | 1 | UniProt (F0NDX3) Pfam (PF03787) In PDB | Sulfolobus islandicus REY15A | |
5 | J | Cmr1,CRISPR-associated RAMP protein, Cmr1 family | polymer | 476 | 55389.0 | 1 | UniProt (F0NDX4) Pfam (PF03787) In PDB | Sulfolobus islandicus REY15A | |
6 | K | CRISPR-associated protein, Cmr2 family | polymer | 1037 | 120856.5 | 1 | UniProt (F0NDX2) Pfam (PF12469) Pfam (PF22335) In PDB | Sulfolobus islandicus REY15A | |
7 | L, M, N, O, P... | CRISPR-associated protein Cmrx | polymer | 174 | 19705.6 | 26 | UniProt (F0NDX7) Pfam (PF19021) In PDB | Sulfolobus islandicus REY15A | |
8 | U | Cognate target RNA | polymer | 46 | 14704.7 | 1 | synthetic construct | ||
9 | V | crRNA | polymer | 51 | 16409.9 | 1 | GenBank (323473489) | Sulfolobus islandicus REY15A | |
10 | K | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
11 | K | PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER | non-polymer | 506.2 | 2 | Chemie (ANP) | |||
12 | K | MANGANESE (II) ION | non-polymer | 54.9 | 3 | Chemie (MN) |
Sequence modifications
D, E, F, G: 1 - 286 (UniProt: F0NDX6)
I: 1 - 283 (UniProt: F0NDX3)
J: 1 - 5 (PDB: 6SH8)
J: 6 - 476 (UniProt: F0NDX4)
V: 1 - 51 (GenBank: 323473489)
PDB | External Database | Details |
---|---|---|
Ala 31 | Asp 31 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Ala 284 | - | expression tag |
Ala 285 | - | expression tag |
Ala 286 | - | expression tag |
His 287 | - | expression tag |
His 288 | - | expression tag |
His 289 | - | expression tag |
His 290 | - | expression tag |
His 291 | - | expression tag |
His 292 | - | expression tag |
His 293 | - | expression tag |
His 294 | - | expression tag |
His 295 | - | expression tag |
His 296 | - | expression tag |
J: 6 - 476 (UniProt: F0NDX4)
V: 1 - 51 (GenBank: 323473489)
PDB | External Database | Details |
---|---|---|
A 1 | C 2068518 | conflict |
U 3 | G 2068520 | conflict |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 39 |
Total formula weight | 972835.2 | |
Non-Polymers* | Number of molecules | 6 |
Total formula weight | 1242.6 | |
All* | Total formula weight | 974077.8 |