6SCD

Polyester hydrolase PE-H Y250S mutant of Pseudomonas aestusnigri

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Polyester hydrolase	polymer	312	33329.0	2	UniProt (A0A1H6AD45)	Pseudomonas aestusnigri
2	C (A)	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
3	D, E, M (A, B)	PHOSPHATE ION	non-polymer		95.0	3	Chemie (PO4)
4	F, N, O (A, B)	SODIUM ION	non-polymer		23.0	3	Chemie (NA)
5	G, H, P (A, B)	CHLORIDE ION	non-polymer		35.5	3	Chemie (CL)
6	I (A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
7	J, K (A)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
8	L (A)	ACETATE ION	non-polymer		59.0	1	Chemie (ACT)
9	Q, R (A, B)	water	water		18.0	493	Chemie (HOH)

Sequence modifications

A, B: 1 - 304 (UniProt: A0A1H6AD45)

PDB	External Database	Details
Ser 250	Tyr 250	engineered mutation
Leu 305	-	expression tag
Glu 306	-	expression tag
His 307	-	expression tag
His 308	-	expression tag
His 309	-	expression tag
His 310	-	expression tag
His 311	-	expression tag
His 312	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	66658.1
Non-Polymers*	Number of molecules	14
	Total formula weight	905.7
All*	Total formula weight	67563.8

*Water molecules are not included.