6SAV

Structural and functional characterisation of three novel fungal amylases with enhanced stability and pH tolerance

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Alpha-amylase	polymer	438	48268.9	1	UniProt (M9TI89) Pfam (PF00128) Pfam (PF09260) In PDB	Rhizomucor pusillus
2	B	Alpha-amylase	polymer	438	48269.8	1	UniProt (M9TI89) Pfam (PF00128) Pfam (PF09260) In PDB	Rhizomucor pusillus
3	A, B	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
4	A, B	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	3	Chemie (NAG)
5	A, B	GLYCEROL	non-polymer		92.1	7	Chemie (GOL)
6	A, B	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
7		water	water		18.0	943	Chemie (HOH)

Sequence modifications

A: 1 - 438 (UniProt: M9TI89)

PDB	External Database	Details
Ala 94	Thr 127	conflict
Asp 103	Gly 136	conflict
Ser 231	Pro 264	conflict
Thr 333	Ala 366	conflict
Ala 358	Thr 391	conflict

B: 1 - 438 (UniProt: M9TI89)

PDB	External Database	Details
Ala 94	Thr 127	conflict
Asp 103	Gly 136	conflict
Ias 120	Asn 153	conflict
Ser 231	Pro 264	conflict
Thr 333	Ala 366	conflict
Ala 358	Thr 391	conflict

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	96538.7
Non-Polymers*	Number of molecules	15
	Total formula weight	1676.6
All*	Total formula weight	98215.3

*Water molecules are not included.