6S91

Cryo-EM structure of the Type III-B Cmr-beta bound to cognate target RNA and AMPPnP, state 2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	CRISPR-associated protein, Cmr5 family	polymer	155	17961.8	3	UniProt (F0NDX5)	Sulfolobus islandicus (strain REY15A)
2	D, E, F, G (D, E, F, G)	CRISPR-associated RAMP protein, Cmr4 family	polymer	286	32322.4	4	UniProt (F0NDX6) Pfam (PF03787)	Sulfolobus islandicus (strain REY15A)
3	H (H)	CRISPR-associated protein, Cmr3 family	polymer	313	36008.5	1	UniProt (F0NDX1) Pfam (PF09700)	Sulfolobus islandicus (strain REY15A)
4	I (I)	CRISPR-associated RAMP protein, Cmr6 family	polymer	296	33945.9	1	UniProt (F0NDX3) Pfam (PF03787)	Sulfolobus islandicus (strain REY15A)
5	J (J)	Cmr1,CRISPR-associated RAMP protein, Cmr1 family	polymer	476	55389.0	1	UniProt (F0NDX4) Pfam (PF03787)	Sulfolobus islandicus REY15A
6	K (K)	CRISPR-associated protein, Cmr2 family	polymer	1037	120856.5	1	UniProt (F0NDX2) Pfam (PF12469) Pfam (PF22335)	Sulfolobus islandicus (strain REY15A)
7	L (U)	Cognate target RNA	polymer	46	14704.7	1		synthetic construct
8	M (V)	crRNA (48-MER)	polymer	51	16409.9	1	GenBank (323473489)	Sulfolobus islandicus REY15A
9	AA, BA, CA, DA, EA... (L, M, N, O, P...)	CRISPR-associated protein Cmrx	polymer	174	19705.6	22	UniProt (F0NDX7) Pfam (PF19021)	Sulfolobus islandicus (strain REY15A)
10	JA (K)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
11	KA, LA (K)	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	2	Chemie (ANP)
12	MA, NA, OA (K)	MANGANESE (II) ION	non-polymer		54.9	3	Chemie (MN)

Sequence modifications

D, E, F, G: 1 - 286 (UniProt: F0NDX6)

PDB	External Database	Details
Ala 31	Asp 31	engineered mutation

I: 1 - 283 (UniProt: F0NDX3)

PDB	External Database	Details
Ala 284	-	expression tag
Ala 285	-	expression tag
Ala 286	-	expression tag
His 287	-	expression tag
His 288	-	expression tag
His 289	-	expression tag
His 290	-	expression tag
His 291	-	expression tag
His 292	-	expression tag
His 293	-	expression tag
His 294	-	expression tag
His 295	-	expression tag
His 296	-	expression tag

J: 1 - 5 (PDB: 6S91)
J: 6 - 476 (UniProt: F0NDX4)
V: 1 - 51 (GenBank: 323473489)

PDB	External Database	Details
A 1	C 2068518	conflict
U 3	G 2068520	conflict

Contents of the asymmetric unit

Polymers	Number of chains	35
	Total formula weight	894012.8
Non-Polymers*	Number of molecules	6
	Total formula weight	1242.6
All*	Total formula weight	895255.4

*Water molecules are not included.