6S8E

Cryo-EM structure of the type III-B Cmr-beta complex bound to non-cognate target RNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C	CRISPR-associated protein, Cmr5 family	polymer	155	17961.8	3	UniProt (F0NDX5) In PDB	Sulfolobus islandicus REY15A
2	D, E, F, G	CRISPR-associated RAMP protein, Cmr4 family	polymer	286	32322.4	4	UniProt (F0NDX6) Pfam (PF03787) In PDB	Sulfolobus islandicus REY15A
3	H	CRISPR-associated protein, Cmr3 family	polymer	313	36008.5	1	UniProt (F0NDX1) Pfam (PF09700) In PDB	Sulfolobus islandicus REY15A
4	I	CRISPR-associated RAMP protein, Cmr6 family	polymer	296	33945.9	1	UniProt (F0NDX3) Pfam (PF03787) In PDB	Sulfolobus islandicus REY15A
5	J	Cmr1,CRISPR-associated RAMP protein, Cmr1 family,CRISPR-associated RAMP protein, Cmr1 family	polymer	476	55389.0	1	UniProt (F0NDX4) Pfam (PF03787) In PDB	Sulfolobus islandicus REY15A
6	K	CRISPR-associated protein, Cmr2 family	polymer	1037	120856.5	1	UniProt (F0NDX2) Pfam (PF12469) Pfam (PF22335) In PDB	Sulfolobus islandicus REY15A
7	U	Non-cognate target RNA	polymer	50	15881.3	1		synthetic construct
8	V	crRNA	polymer	51	16409.9	1		Sulfolobus islandicus REY15A
9	X, x, T, W, t...	CRISPR-associated protein Cmr7	polymer	174	19705.6	22	UniProt (F0NDX7) Pfam (PF19021) In PDB	Sulfolobus islandicus REY15A
10	K	ZINC ION	non-polymer		65.4	1	Chemie (ZN)

Sequence modifications

D, E, F, G: 1 - 286 (UniProt: F0NDX6)

PDB	External Database	Details
Ala 31	Asp 31	engineered mutation

I: 1 - 283 (UniProt: F0NDX3)

PDB	External Database	Details
Ala 284	-	expression tag
Ala 285	-	expression tag
Ala 286	-	expression tag
His 287	-	expression tag
His 288	-	expression tag
His 289	-	expression tag
His 290	-	expression tag
His 291	-	expression tag
His 292	-	expression tag
His 293	-	expression tag
His 294	-	expression tag
His 295	-	expression tag
His 296	-	expression tag

J: 1 - 5 (PDB: 6S8E)
J: 6 - 476 (UniProt: F0NDX4)

Contents of the asymmetric unit

Polymers	Number of chains	35
	Total formula weight	895189.4
Non-Polymers*	Number of molecules	1
	Total formula weight	65.4
All*	Total formula weight	895254.8

*Water molecules are not included.