6S84

TsaBDE complex from Thermotoga maritima

Replaces:  6FPE

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, D)	tRNA N6-adenosine threonylcarbamoyltransferase	polymer	327	35672.2	2	UniProt (Q9WXZ2) Pfam (PF00814)	Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)	N6-L-threonylcarbamoyladenine synthase,t(6)A synthase,t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaD,tRNA threonylcarbamoyladenosine biosynthesis protein TsaD
2	B, F (E, B)	ATPase YjeE, predicted to have essential role in cell wall biosynthesis	polymer	184	21513.5	2	UniProt (R4NRX5)	Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
3	D, E (C, F)	tRNA threonylcarbamoyladenosine biosynthesis protein TsaB	polymer	206	23020.8	2	UniProt (Q9WZX7) Pfam (PF00814)	Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)	t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaB
4	G, I, J, M (A, E, D, B)	DIPHOSPHOMETHYLPHOSPHONIC ACID ADENOSYL ESTER	non-polymer		505.2	4	Chemie (APC)
5	H, L (E, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6	K (B)	LEUCINE	non-polymer		131.2	1	Chemie (LEU)
7	N, O, P, Q, R (A, D, C, F, B)	water	water		18.0	18	Chemie (HOH)

Sequence modifications

E, B: -7 - 161 (UniProt: R4NRX5)

PDB	External Database	Details
Met -22	-	initiating methionine
Gly -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
Glu -14	-	expression tag
Asn -13	-	expression tag
Leu -12	-	expression tag
Tyr -11	-	expression tag
Phe -10	-	expression tag
Gln -9	-	expression tag
Gly -8	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	160413.1
Non-Polymers*	Number of molecules	7
	Total formula weight	2200.6
All*	Total formula weight	162613.7

*Water molecules are not included.