6S7T
Cryo-EM structure of human oligosaccharyltransferase complex OST-B
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B | polymer | 826 | 93781.0 | 1 | UniProt (Q8TCJ2) Pfam (PF02516) Pfam (PF21436) | Homo sapiens (Human) | STT3-B,Source of immunodominant MHC-associated peptides homolog |
| 2 | B (B) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4 | polymer | 37 | 4196.0 | 1 | UniProt (P0C6T2) Pfam (PF10215) | Homo sapiens (Human) | |
| 3 | C (C) | Transmembrane protein 258 | polymer | 79 | 9083.8 | 1 | UniProt (P61165) Pfam (PF05251) | Homo sapiens (Human) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit TMEM258,Oligosaccharyl transferase subunit TMEM258 |
| 4 | D (D) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 | polymer | 113 | 12503.6 | 1 | UniProt (P61803) Pfam (PF02109) | Homo sapiens (Human) | Oligosaccharyl transferase subunit DAD1,Defender against cell death 1,DAD-1 |
| 5 | E (E) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 | polymer | 607 | 68656.2 | 1 | UniProt (P04843) Pfam (PF04597) | Homo sapiens (Human) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit,Ribophorin I,RPN-I,Ribophorin-1 |
| 6 | F (F) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2 | polymer | 631 | 69347.5 | 1 | UniProt (P04844) Pfam (PF25147) Pfam (PF05817) Pfam (PF23861) Pfam (PF23860) | Homo sapiens (Human) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa subunit,RIBIIR,Ribophorin II,RPN-II,Ribophorin-2 |
| 7 | G (G) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit | polymer | 456 | 50754.4 | 1 | UniProt (A0A024RAD5) Pfam (PF03345) Pfam (PF23358) UniProt (by SIFTS) (P39656) | Homo sapiens (Human) | Oligosaccharyl transferase 48 kDa subunit |
| 8 | H (H) | Magnesium transporter protein 1 | polymer | 335 | 38081.6 | 1 | UniProt (Q9H0U3) Pfam (PF04756) | Homo sapiens (Human) | MagT1,Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit MAGT1,Oligosaccharyl transferase subunit MAGT1,Implantation-associated protein,IAP |
| 9 | I (I) | Malectin | polymer | 292 | 32270.8 | 1 | UniProt (Q14165) Pfam (PF11721) | Homo sapiens (Human) | |
| 10 | J (K) | PEPTIDE | polymer | 7 | 588.6 | 1 | Homo sapiens (Human) | ||
| 11 | K, M (J, M) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 2 | In PDB GlyTouCan (G42666HT) | |||
| 12 | L (L) | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1397.2 | 1 | In PDB GlyTouCan (G81980VO) | |||
| 13 | N (N) | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1397.2 | 1 | In PDB GlyTouCan (G80966KZ) | |||
| 14 | AA, EA, IA, O, P... (D, E, F, A, C) | (4R,7R)-4-hydroxy-N,N,N-trimethyl-4,9-dioxo-7-[(undecanoyloxy)methyl]-3,5,8-trioxa-4lambda~5~-phosphadocosan-1-aminium | non-polymer | 636.9 | 10 | Chemie (EGY) | |||
| 15 | BA, CA, DA, FA, GA... (D, E, F, G, A...) | (2~{S},3~{R},4~{R},5~{S},6~{S})-2-(hydroxymethyl)-6-[(1~{S},2~{R},3~{R},4~{R},5'~{S},6~{S},7~{R},8~{S},9~{R},12~{R},13~{R},15~{S},16~{S},18~{R})-5',7,9,13-tetramethyl-3,15-bis(oxidanyl)spiro[5-oxapentacyclo[10.8.0.0^{2,9}.0^{4,8}.0^{13,18}]icosane-6,2'-oxane]-16-yl]oxy-oxane-3,4,5-triol | non-polymer | 610.8 | 13 | Chemie (KZB) | |||
| 16 | HA, V (E, A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 17 | W (A) | (2Z,6Z,10Z,14Z,18Z,22Z,26Z)-3,7,11,15,19,23,27,31-octamethyldotriaconta-2,6,10,14,18,22,26,30-octaen-1-yl dihydrogen phosphate | non-polymer | 642.9 | 1 | Chemie (0K3) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 379263.5 | |
| Branched | Number of molecules | 4 |
| Total formula weight | 3643.3 | |
| Non-Polymers* | Number of molecules | 26 |
| Total formula weight | 15000.2 | |
| All* | Total formula weight | 397907.1 |
