6S7T

Cryo-EM structure of human oligosaccharyltransferase complex OST-B

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B	polymer	826	93781.0	1	UniProt (Q8TCJ2) Pfam (PF02516) Pfam (PF21436) In PDB	Homo sapiens (Human)	STT3-B,Source of immunodominant MHC-associated peptides homolog
2	B	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4	polymer	37	4196.0	1	UniProt (P0C6T2) Pfam (PF10215) In PDB	Homo sapiens (Human)
3	C	Transmembrane protein 258	polymer	79	9083.8	1	UniProt (P61165) Pfam (PF05251) In PDB	Homo sapiens (Human)	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit TMEM258,Oligosaccharyl transferase subunit TMEM258
4	D	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1	polymer	113	12503.6	1	UniProt (P61803) Pfam (PF02109) In PDB	Homo sapiens (Human)	Oligosaccharyl transferase subunit DAD1,Defender against cell death 1,DAD-1
5	E	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1	polymer	607	68656.2	1	UniProt (P04843) Pfam (PF04597) In PDB	Homo sapiens (Human)	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit,Ribophorin I,RPN-I,Ribophorin-1
6	F	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2	polymer	631	69347.5	1	UniProt (P04844) Pfam (PF05817) In PDB	Homo sapiens (Human)	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa subunit,RIBIIR,Ribophorin II,RPN-II,Ribophorin-2
7	G	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit	polymer	456	50754.4	1	UniProt (A0A024RAD5) Pfam (PF03345) UniProt (by SIFTS) (P39656) In PDB	Homo sapiens (Human)	Oligosaccharyl transferase 48 kDa subunit
8	H	Magnesium transporter protein 1	polymer	335	38081.6	1	UniProt (Q9H0U3) Pfam (PF04756) In PDB	Homo sapiens (Human)	MagT1,Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit MAGT1,Oligosaccharyl transferase subunit MAGT1,Implantation-associated protein,IAP
9	I	Malectin	polymer	292	32270.8	1	UniProt (Q14165) Pfam (PF11721) In PDB	Homo sapiens (Human)
10	K	PEPTIDE	polymer	7	588.6	1		Homo sapiens (Human)
11	J, M	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
12	L	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1397.2	1	In PDB GlyTouCan (G81980VO)
13	N	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1397.2	1	In PDB GlyTouCan (G80966KZ)
14	D, E, F, A, C	(4R,7R)-4-hydroxy-N,N,N-trimethyl-4,9-dioxo-7-[(undecanoyloxy)methyl]-3,5,8-trioxa-4lambda~5~-phosphadocosan-1-aminium	non-polymer		636.9	10	Chemie (EGY)
15	D, E, F, G, A...	(2~{S},3~{R},4~{R},5~{S},6~{S})-2-(hydroxymethyl)-6-[(1~{S},2~{R},3~{R},4~{R},5'~{S},6~{S},7~{R},8~{S},9~{R},12~{R},13~{R},15~{S},16~{S},18~{R})-5',7,9,13-tetramethyl-3,15-bis(oxidanyl)spiro[5-oxapentacyclo[10.8.0.0^{2,9}.0^{4,8}.0^{13,18}]icosane-6,2'-oxane]-16-yl]oxy-oxane-3,4,5-triol	non-polymer		610.8	13	Chemie (KZB)
16	E, A	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
17	A	(2Z,6Z,10Z,14Z,18Z,22Z,26Z)-3,7,11,15,19,23,27,31-octamethyldotriaconta-2,6,10,14,18,22,26,30-octaen-1-yl dihydrogen phosphate	non-polymer		642.9	1	Chemie (0K3)

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	379263.5
Branched	Number of molecules	4
	Total formula weight	3643.3
Non-Polymers*	Number of molecules	26
	Total formula weight	15000.2
All*	Total formula weight	397907.1

*Water molecules are not included.