6S72

Crystal Structure of the Homospermidine Synthase (HSS) variant W229A from Blastochloris viridis in Complex with NAD and PUT

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Homospermidine synthase	polymer	480	52922.4	2	UniProt (O32323) Pfam (PF03435) Pfam (PF16653)	Blastochloris viridis	HSS
2	C, E (A, B)	NICOTINAMIDE-ADENINE-DINUCLEOTIDE	non-polymer		663.4	2	Chemie (NAD)
3	D (A)	1,4-DIAMINOBUTANE	non-polymer		88.2	1	Chemie (PUT)
4	F, G (A, B)	water	water		18.0	863	Chemie (HOH)

Sequence modifications

A, B: 2 - 477 (UniProt: O32323)

PDB	External Database	Details
Gly -2	-	expression tag
Pro -1	-	expression tag
Met 0	-	expression tag
Gly 1	-	expression tag
Ala 229	Trp 229	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	105844.7
Non-Polymers*	Number of molecules	3
	Total formula weight	1415.0
All*	Total formula weight	107259.7

*Water molecules are not included.