6S6Q

Crystal structure of the LRR ectodomain of the plant membrane receptor kinase GASSHO1/SCHENGEN3 from Arabidopsis thaliana in complex with CASPARIAN STRIP INTEGRITY FACTOR 2.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	LRR receptor-like serine/threonine-protein kinase GSO1	polymer	863	94177.6	2	UniProt (C0LGQ5) Pfam (PF08263) Pfam (PF00560) Pfam (PF23598) Pfam (PF13855)	Arabidopsis thaliana (Mouse-ear cress)	Protein GASSHO 1,Protein SCHENGEN 3
2	C, D (C, D)	Protein CASPARIAN STRIP INTEGRITY FACTOR 2	polymer	21	2475.9	2	UniProt (O65684)	Arabidopsis thaliana
3	E, F, G, K (E, F, G, K)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	4	In PDB GlyTouCan (G42666HT)
4	H, J (H, J)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	2	In PDB GlyTouCan (G15407YE)
5	I (I)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		383.3	1	In PDB GlyTouCan (G90333CG)
6	AA, BA, CA, DA, EA... (B, A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	20	Chemie (NAG)

Sequence modifications

A, B: 18 - 870 (UniProt: C0LGQ5)

PDB	External Database	Details
Gly 14	-	expression tag
Ser 15	-	expression tag
Ser 16	-	expression tag
Met 17	-	expression tag
Glu 871	-	expression tag
Asn 872	-	expression tag
Leu 873	-	expression tag
Tyr 874	-	expression tag
Phe 875	-	expression tag
Gln 876	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	193307.0
Branched	Number of molecules	7
	Total formula weight	3254.0
Non-Polymers*	Number of molecules	20
	Total formula weight	4424.2
All*	Total formula weight	200985.2

*Water molecules are not included.