6S65

Crystal Structure of the Homospermidine Synthase (HSS) variant N135F from Blastochloris viridis in Complex with NAD

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Homospermidine synthase	polymer	480	53070.6	2	UniProt (O32323) Pfam (PF03435) Pfam (PF16653)	Blastochloris viridis	HSS
2	C, D (A, B)	NICOTINAMIDE-ADENINE-DINUCLEOTIDE	non-polymer		663.4	2	Chemie (NAD)
3	E, F (A, B)	water	water		18.0	599	Chemie (HOH)

Sequence modifications

A, B: 2 - 477 (UniProt: O32323)

PDB	External Database	Details
Gly -2	-	expression tag
Pro -1	-	expression tag
Met 0	-	expression tag
Gly 1	-	expression tag
Phe 135	Asn 135	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	106141.1
Non-Polymers*	Number of molecules	2
	Total formula weight	1326.8
All*	Total formula weight	107468.0

*Water molecules are not included.