6S48
AvaII RESTRICTION ENDONUCLEASE IN COMPLEX WITH PARTIALLY CLEAVED dsDNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Type II site-specific deoxyribonuclease | polymer | 238 | 27161.8 | 2 | UniProt (Q8YYB7) Pfam (PF09570) | Nostoc sp. PCC 7120 = FACHB-418 | AvaII restriction endonuclease |
| 2 | C (C) | DNA (5'-D(*GP*AP*TP*GP*GP*TP*CP*CP*TP*AP*C)-3') | polymer | 11 | 3349.2 | 1 | synthetic construct | ||
| 3 | D, H (D, H) | DNA (5'-D(*GP*TP*AP*G)-3') | polymer | 4 | 1230.9 | 2 | synthetic construct | ||
| 4 | E, I (E, I) | DNA (5'-D(P*GP*AP*CP*CP*AP*TP*C)-3') | polymer | 7 | 2082.4 | 2 | synthetic construct | ||
| 5 | F (F) | DNA (5'-D(*GP*AP*TP*G)-3') | polymer | 4 | 1230.9 | 1 | synthetic construct | ||
| 6 | G (G) | DNA (5'-D(P*GP*TP*CP*CP*TP*AP*C)-3') | polymer | 7 | 2073.4 | 1 | synthetic construct | ||
| 7 | J, L, M, R, S (A, B, C, E) | CALCIUM ION | non-polymer | 40.1 | 5 | Chemie (CA) | |||
| 8 | K, P (A, B) | BETA-MERCAPTOETHANOL | non-polymer | 78.1 | 2 | Chemie (BME) | |||
| 9 | N (B) | PENTAETHYLENE GLYCOL | non-polymer | 238.3 | 1 | Chemie (1PE) | |||
| 10 | O (B) | TRIETHYLENE GLYCOL | non-polymer | 150.2 | 1 | Chemie (PGE) | |||
| 11 | Q (B) | SERINE | non-polymer | 105.1 | 1 | Chemie (SER) | |||
| 12 | AA, T, U, V, W... (I, A, B, C, D...) | water | water | 18.0 | 487 | Chemie (HOH) |
Sequence modifications
A, B: 3 - 230 (UniProt: Q8YYB7)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Gly 2 | - | expression tag |
| Leu 231 | - | expression tag |
| Glu 232 | - | expression tag |
| His 233 | - | expression tag |
| His 234 | - | expression tag |
| His 235 | - | expression tag |
| His 236 | - | expression tag |
| His 237 | - | expression tag |
| His 238 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 9 |
| Total formula weight | 67603.5 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 850.2 | |
| All* | Total formula weight | 68453.7 |
