6S45

Room temperature structure of the dark state of the LOV2 domain of phototropin-2 from Arabidopsis thaliana determined with a serial crystallography approach

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Phototropin-2	polymer	128	15007.9	1	UniProt (P93025) Pfam (PF13426)	Arabidopsis thaliana (thale cress)	Defective in chloroplast avoidance protein 1,Non-phototropic hypocotyl 1-like protein 1,NPH1-like protein 1
2	B (A)	FLAVIN MONONUCLEOTIDE	non-polymer		456.3	1	Chemie (FMN)
3	C (A)	water	water		18.0	42	Chemie (HOH)

Sequence modifications

A: 388 - 492 (UniProt: P93025)

PDB	External Database	Details
Met 387	-	initiating methionine
Glu 493	-	expression tag
Phe 494	-	expression tag
Ile 495	-	expression tag
Pro 496	-	expression tag
Asn 497	-	expression tag
Pro 498	-	expression tag
Leu 499	-	expression tag
Leu 500	-	expression tag
Gly 501	-	expression tag
Leu 502	-	expression tag
Asp 503	-	expression tag
Ser 504	-	expression tag
Thr 505	-	expression tag
Arg 506	-	expression tag
Thr 507	-	expression tag
Gly 508	-	expression tag
His 509	-	expression tag
His 510	-	expression tag
His 511	-	expression tag
His 512	-	expression tag
His 513	-	expression tag
His 514	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	15007.9
Non-Polymers*	Number of molecules	1
	Total formula weight	456.3
All*	Total formula weight	15464.3

*Water molecules are not included.