6S42

The double mutant(Ile44Leu+Gln102His) of haloalkane dehalogenase DbeA from Bradyrhizobium elkanii USDA94 with an eliminated halide-binding site

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Haloalkane dehalogenase	polymer	310	33992.7	1	UniProt (E2RV62) Pfam (PF00561) In PDB	Bradyrhizobium elkanii
2	A	HEXANE-1,6-DIOL	non-polymer		118.2	1	Chemie (HEZ)
3	A	IODIDE ION	non-polymer		126.9	8	Chemie (IOD)
4	A	CHLORIDE ION	non-polymer		35.5	3	Chemie (CL)
5		water	water		18.0	469	Chemie (HOH)

Sequence modifications

A: 1 - 302 (UniProt: E2RV62)

PDB	External Database	Details
Leu 44	Ile 44	engineered mutation
His 102	Gln 102	engineered mutation
Leu 303	-	expression tag
Glu 304	-	expression tag
His 305	-	expression tag
His 306	-	expression tag
His 307	-	expression tag
His 308	-	expression tag
His 309	-	expression tag
His 310	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	33992.7
Non-Polymers*	Number of molecules	12
	Total formula weight	1239.8
All*	Total formula weight	35232.4

*Water molecules are not included.