6S3Z

Structure Of D80A-Fructofuranosidase From Xanthophyllomyces Dendrorhous Complexed With Fructose And hydroquinone

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Beta-fructofuranosidase	polymer	665	72007.1	2	UniProt (J7HDY4) Pfam (PF00251) Pfam (PF08244)	Phaffia rhodozyma (Yeast)
2	C, F (C, F)	alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1073.0	2	In PDB GlyTouCan (G09724ZC)
3	D, G (D, G)	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1721.5	2	In PDB GlyTouCan (G40702WU)
4	E, H (E, H)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
5	CA, I (B, A)	beta-D-fructofuranose	non-polymer		180.2	2	Chemie (FRU)
6	DA, J (B, A)	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
7	EA, FA, GA, K, L... (B, A)	1,4-benzoquinone	non-polymer		108.1	6	Chemie (PLQ)
8	AA, BA, HA, IA, JA... (A, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	26	Chemie (NAG)
9	SA, TA (A, B)	water	water		18.0	971	Chemie (HOH)

Sequence modifications

A, B: 1 - 665 (UniProt: J7HDY4)

PDB	External Database	Details
Val 2	Ile 2	conflict
Ala 80	Asp 80	engineered mutation
Ala 663	Ser 663	conflict
Tyr 665	Arg 665	conflict

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	144014.3
Branched	Number of molecules	6
	Total formula weight	6437.8
Non-Polymers*	Number of molecules	36
	Total formula weight	6884.4
All*	Total formula weight	157336.5

*Water molecules are not included.