6S3J

Crystal Structure of lipase from Geobacillus stearothermophilus T6 variant E134C/F149C

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Lipase	polymer	392	43863.0	1	UniProt (Q93A71) In PDB	Geobacillus stearothermophilus
2	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
3	A	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
4		water	water		18.0	343	Chemie (HOH)

Sequence modifications

A: 4 - 389 (UniProt: Q93A71)

PDB	External Database	Details
Cys 134	Glu 163	engineered mutation
Cys 149	Phe 178	engineered mutation
Ala 323	Thr 352	conflict
His 390	-	expression tag
His 391	-	expression tag
His 392	-	expression tag
His 393	-	expression tag
His 394	-	expression tag
His 395	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	43863.0
Non-Polymers*	Number of molecules	2
	Total formula weight	105.5
All*	Total formula weight	43968.5

*Water molecules are not included.