6S31

Crystal structure of ene-reductase GsOYE from Galdieria sulphuraria in complex with 4-Hydroxybenzaldehyde

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	NADPH2 dehydrogenase-like protein	polymer	400	45381.0	1	UniProt (M2XAQ9) Pfam (PF00724)	Galdieria sulphuraria (Red alga)
2	B, C, D (A)	P-HYDROXYBENZALDEHYDE	non-polymer		122.1	3	Chemie (HBA)
3	E (A)	FLAVIN MONONUCLEOTIDE	non-polymer		456.3	1	Chemie (FMN)
4	F (A)	water	water		18.0	447	Chemie (HOH)

Sequence modifications

A: 1 - 380 (UniProt: M2XAQ9)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 204	Thr 204	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	45381.0
Non-Polymers*	Number of molecules	4
	Total formula weight	822.7
All*	Total formula weight	46203.8

*Water molecules are not included.