6S23

Crystal structure of ene-reductase GsOYE from Galleria sulphuraria in complex with 2-methyl-cyclopenten-1-one

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	NADPH2 dehydrogenase-like protein	polymer	401	45494.2	2	UniProt (M2XAQ9) Pfam (PF00724)	Galdieria sulphuraria (Red alga)
2	C, F (A, B)	FLAVIN MONONUCLEOTIDE	non-polymer		456.3	2	Chemie (FMN)
3	D, E, G (A, B)	2-methylcyclopenta-2,4-dien-1-one	non-polymer		94.1	3	Chemie (KSW)
4	H (B)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
5	I, J (A, B)	water	water		18.0	96	Chemie (HOH)

Sequence modifications

A, B: 1 - 381 (UniProt: M2XAQ9)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 204	Thr 204	conflict

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	90988.4
Non-Polymers*	Number of molecules	6
	Total formula weight	1219.3
All*	Total formula weight	92207.7

*Water molecules are not included.