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6S0W

The crystal structure of kanamycin B dioxygenase (KanJ) from Streptomyces kanamyceticus in complex with nickel and kanamycin B sulfate

Entity
Entity IDChain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
1A, B, C, D, E...Kanamycin B dioxygenasepolymer28831841.16UniProt (Q6L732)
Pfam (PF05721)
In PDB
Streptomyces kanamyceticusKanamycin biosynthesis protein J
2E, F, A, B, C...(1R,2S,3S,4R,6S)-4,6-DIAMINO-3-[(3-AMINO-3-DEOXY-ALPHA-D-GLUCOPYRANOSYL)OXY]-2-HYDROXYCYCLOHEXYL 2,6-DIAMINO-2,6-DIDEOXY-ALPHA-D-GLUCOPYRANOSIDEnon-polymer483.56Chemie (9CS)
3E, F, A, B, C...NICKEL (II) IONnon-polymer58.76Chemie (NI)
4E, F, A, B, C...SULFATE IONnon-polymer96.119Chemie (SO4)
5DDI(HYDROXYETHYL)ETHERnon-polymer106.11Chemie (PEG)
6waterwater18.01007Chemie (HOH)
Sequence modifications
A, B, C, D, E, F: 1 - 285 (UniProt: Q6L732)
PDBExternal DatabaseDetails
Ser -2-expression tag
Asn -1-expression tag
Ala 0-expression tag
Sequence viewer
Contents of the asymmetric unit
PolymersNumber of chains6
Total formula weight191046.6
Non-Polymers*Number of molecules32
Total formula weight5184.6
All*Total formula weight196231.2
*Water molecules are not included.

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PDB entries from 2024-07-31

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