6S06
Crystal structure of haloalkane dehalogenase LinB D147C+L177C mutant (LinB73) from Sphingobium japonicum UT26
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Haloalkane dehalogenase | polymer | 302 | 33951.5 | 1 | UniProt (D4Z2G1) Pfam (PF00561) | Sphingobium japonicum (strain DSM 16413 / CCM 7287 / MTCC 6362 / UT26 / NBRC 101211 / UT26S) | 1,3,4,6-tetrachloro-1,4-cyclohexadiene halidohydrolase,4-TCDN halidohydrolase |
| 2 | B (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 3 | C, D, E (A) | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
| 4 | F (A) | water | water | 18.0 | 233 | Chemie (HOH) |
Sequence modifications
A: 1 - 296 (UniProt: D4Z2G1)
| PDB | External Database | Details |
|---|---|---|
| Cys 147 | Asp 147 | engineered mutation |
| Cys 177 | Leu 177 | engineered mutation |
| His 297 | - | expression tag |
| His 298 | - | expression tag |
| His 299 | - | expression tag |
| His 300 | - | expression tag |
| His 301 | - | expression tag |
| His 302 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 33951.5 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 108.4 | |
| All* | Total formula weight | 34059.9 |
