6S06

Crystal structure of haloalkane dehalogenase LinB D147C+L177C mutant (LinB73) from Sphingobium japonicum UT26

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Haloalkane dehalogenase	polymer	302	33951.5	1	UniProt (D4Z2G1) Pfam (PF00561) In PDB	Sphingobium japonicum (strain DSM 16413 / CCM 7287 / MTCC 6362 / UT26 / NBRC 101211 / UT26S)	1,3,4,6-tetrachloro-1,4-cyclohexadiene halidohydrolase,4-TCDN halidohydrolase
2	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
3	A	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
4		water	water		18.0	233	Chemie (HOH)

Sequence modifications

A: 1 - 296 (UniProt: D4Z2G1)

PDB	External Database	Details
Cys 147	Asp 147	engineered mutation
Cys 177	Leu 177	engineered mutation
His 297	-	expression tag
His 298	-	expression tag
His 299	-	expression tag
His 300	-	expression tag
His 301	-	expression tag
His 302	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	33951.5
Non-Polymers*	Number of molecules	4
	Total formula weight	108.4
All*	Total formula weight	34059.9

*Water molecules are not included.