6RXA

EDDS lyase variant D290M/Y320M with bound formate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Argininosuccinate lyase	polymer	508	55742.4	1	UniProt (Q11KV9) Pfam (PF00206) Pfam (PF14698) In PDB	Chelativorans sp. (strain BNC1)
2	A	FORMIC ACID	non-polymer		46.0	3	Chemie (FMT)
3	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
4	A	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
5		water	water		18.0	238	Chemie (HOH)

Sequence modifications

A: 1 - 502 (UniProt: Q11KV9)

PDB	External Database	Details
Met 290	Asp 290	engineered mutation
Met 320	Tyr 320	engineered mutation
His 503	-	expression tag
His 504	-	expression tag
His 505	-	expression tag
His 506	-	expression tag
His 507	-	expression tag
His 508	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	55742.4
Non-Polymers*	Number of molecules	6
	Total formula weight	276.1
All*	Total formula weight	56018.5

*Water molecules are not included.